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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1981Articles/Phyto71n12_1303.PDF Last checked: 08/16/2012 Requires: PDF Reader |
Publication Type:
| Refereed Report |
Author(s): | Liao, C. H.;
Chen, T. A. |
Author Affiliation: | Liao: Assistant Research Professor; Chen: Professor, Department of Plant Pathology, Cook College, Rutgers University, New Brunswick, NJ |
Title: | Isolation, culture and pathogenicity to Sudan grass of a Corynebacterium associated with ratoon stunting of sugarcane and with Bermuda grass |
Section: | Etiology Other records with the "Etiology" Section
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Source: | Phytopathology. Vol. 71, No. 12, December 1981, p. 1303-1306. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 4 |
Related Web URL: | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1981Abstracts/Phyto71_1303.htm Last checked: 08/16/2012 Notes: Abstract only |
Keywords: | TIC Keywords: Cynodon dactylon; Genetics; Growth factors; Pathogenicity; Sorghum sudanense; Wilting
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Abstract/Contents: | "The small bacterium associated with ratoon stunting disease (RSD) of sugarcane was successfully cultured in a cellfree medium containing Muller-Hinton agar (3.8%), or Muller-Hinton broth (2.2%), hemoglobin (10 mĪ¼ g/ml), Isovitalex (BBL) (1%), asparagine (0.2%), glutamic acid (0.05%), KH2PO4 (0.1%), K2HPO4 (0.25%), MgSO4.7H2O (0.02%), sodium citrate (0.01%), ammonium sulphate (0.05%), CaCl2 (0.5 mĪ¼ g/ml), ZnSO4 (0.01 mĪ¼ g/ml) and CuSO4 (0.01 mĪ¼ g/ml). The same medium was also used to isolate and culture the RSD-like bacterium present in Bermuda grass. The bacterium recovered from either sugarcane or Bermuda grass can infect and multiply in a Sudan grass-sorghum hybrid. Wilting symptoms were observed in some of the inoculated plants. Cultured organisms exhibited lack of motility, absence of endospores, nonacid fastness, and Grampositive reaction but often irregular staining, and aerobic growth; a filamentous-rod (0.25-0.35 X 2-5 Ī¼m), clublike, or occasionally V-form morphology was observed. Tiny colonies (0.05-0.23 mm diameter) were observed in agar medium after 8-19 days of incubation at 30 C." |
Language: | English |
References: | 22 |
Note: | Pictures, b/w Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Liao, C. H., and T. A. Chen. 1981. Isolation, culture and pathogenicity to Sudan grass of a Corynebacterium associated with ratoon stunting of sugarcane and with Bermuda grass. Phytopathology. 71(12):p. 1303-1306. |
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| MSU catalog number: b2219736a |
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