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| Web URL(s): | https://www.crops.org/publications/cs/pdfs/39/3/CS0390030796
Last checked: 08/05/2010
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Hiatt, E. E. III;
Hill, N. S.;
Bouton, J. H.;
Stuedemann, J. A. |
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| Author Affiliation: | Hiatt, Hill, Bouton: Department of Crop and Soil Sciences, University of Georgia, Athens GA; Stuedemann: USDA-ARS, J. Phil Campbell, Sr. Natural Resouce Conservation Center, Watkinsville, GA |
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| Title: | Tall fescue endophyte detection: Commerical immunoblot test kit compared with microscopic analysis |
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| Section: | Crop quality & utilization
Other records with the "Crop quality & utilization" Section
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| Source: | Crop Science. Vol. 39, No. 3, May/June 1999, p. 796-799. |
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| Publishing Information: | Madison, WI: Crop Science Society of America |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: Festuca arundinacea; Acremonium coenophialum; Testing; Techniques; Tissue immunoblot; Microscopy; Comparisons
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| Abstract/Contents: | "A reliable, efficient, and accurate detection method for presence of the endophytic fungus Neotyphodium coenophialum (Morgan-Jones and Gams) Glenn, Bacon, and Hanlin comb. nov. in tall fescue (Festuca arundinacea Schreb.) seed and plant tissue would be beneficial for tall fescue breeding and seed lot analysis. This experiment was conducted to determine the accuracy, reliability, and reproducibility of the Phytoscreen Neotyphodium immunoblot detection kit (Agrinostics Ltd. Co., Watkinsville, GA). Tissue immunoblot was compared with histological staining followed by microscopic analysis on tall fescue tillers from a greenhouse grow-out test, field grown spaced plants, established field paddocks, and different tall fescue seed lots. Endophyte-infected and endophyte-free tall fescue populations were evaluated by both methods. Results obtained by both methods were similar regardless of the infection level of the population, type of tisse assayed, or the technician that conducted the assay. The immunoblot detection kit was accurate and reliable and readily accomodated large numbers of samples." |
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| Language: | English |
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| References: | 12 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hiatt, E. E. III, N. S. Hill, J. H. Bouton, and J. A. Stuedemann. 1999. Tall fescue endophyte detection: Commerical immunoblot test kit compared with microscopic analysis. Crop Sci. 39(3):p. 796-799. |
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| Web URL(s):
https://www.crops.org/publications/cs/pdfs/39/3/CS0390030796
Last checked: 08/05/2010
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: SB 183 .C7 |
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