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| Web URL(s): | https://academic.oup.com/jee/article/92/5/1079/2217101/Selection-of-a-Nucleopolyhedrovirus-for-Control-of
Last checked: 02/28/2017
Access conditions: Item is within a limited-access website
Notes: Guide page |
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| Publication Type:
| Refereed |
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| Author(s): | Escribano, Ana;
Williams, Trevor;
Goulson, David;
Cave, Ronald D.;
Chapman, Jason W.;
Caballero, Primitivo |
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| Author Affiliation: | Escribano and Caballero: Laboratorio de Entomologia Agricola y Patologia de Insectos, Departmento de Produccion Agraria, Universidad Publica de Navarra, Spain; Williams: ECOSUR, Chiapas, Mexico; Goulson and Chapman: Division of Biodiversity and Ecology, School of Biological Sciences, University of Southampton, UK; Cave: Departmento de Proteccion Vegetal, Escuela Agricola Panamericana, Honduras |
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| Title: | Selection of a nucleopolyhedrovirus for control of Spodoptera frugiperda (Lepidoptera: Noctuidae): Structural, genetic, and biological comparison of four isolates from the Americas |
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| Section: | Biological and microbial control
Other records with the "Biological and microbial control" Section
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| Source: | Journal of Economic Entomology. Vol. 92, No. 5, October 1999, p. 1079-1085. |
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| Publishing Information: | Lanham, MD: Entomological Society of America |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Spodoptera frugiperda; Pest control; Genetics; Biological control
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| Abstract/Contents: | "Spodoptera frugiperda (J.E. Smith)(Lepidoptera: Noctuidae) is the principal pest of maize in tropical and subtropical regions of the Americas. Larvae of this species are susceptible to a nucleopolyhedrovirus (NPV) which has attacked interest as a potential biocontrol agent. Four strains of NPV isolated from infected S. frugiperda larvae in the United States, Nicaragua, and Argentina were subjected to a structural, genetic, and biological comparison to select a candidate isolate for use in biocontrol experiments in Mexico and Honduras. All isolates had an occlusion body polyhedrin protein of 32 kDa, but the virions of each isolate differed subtly in the pattern and abundance of certain structural polypeptides revealed by SDS-PAGE analysis. Restriction endonuclease analysis of viral DNA confirmed that these isolates were strains of a single virus species but showed that they were not genetically homogenous; each isolate could be differentiated from the others using common restriction enzymes. Droplet feeding bioassays indicated that an isolate from Nicaragua (Sf-NIC) and an isolate from the United States (Sf-US) had the highest infectivity when tested against 2nd instars originating from a Honduran S. frugiperda colony. No significant differences were detected in the speed of kill of Sf-NIC (102.7 h), Sf-US (102.3 h), and Sf-AR (103.4 h), whereas that of Sf-2 (97.3 h) was significantly shorter. Additional bioassays of the Sf-NIC isolate against 2nd to 6th instars demonstrated that LCā
ā values increased with larval stage from 2.03 x 10āµ OBs/ml for 2nd instars to 1.84 x 10āø OBs/ml for 5th instars. The concentration required to elicit a lethal infection of 6th instars was so high that a reliable estimate of LCā
ā could not be obtained. The mean time to death for each stage challenged with the Sf-NIC isolate increased with instar from an average of 102.7 h in 2nd instars to 136.9 h in 5th instars." |
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| Language: | English |
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| References: | 45 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Escribano, A., T. Williams, D. Goulson, R. D. Cave, J. W. Chapman, and P. Caballero. 1999. Selection of a nucleopolyhedrovirus for control of Spodoptera frugiperda (Lepidoptera: Noctuidae): Structural, genetic, and biological comparison of four isolates from the Americas. J. Econ. Entomol. 92(5):p. 1079-1085. |
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| Web URL(s):
https://academic.oup.com/jee/article/92/5/1079/2217101/Selection-of-a-Nucleopolyhedrovirus-for-Control-of
Last checked: 02/28/2017
Access conditions: Item is within a limited-access website
Notes: Guide page |
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MSU catalog number: SB 931 .A1 J6 |
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