Full TGIF Record # 62724
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Web URL(s):http://www.tandfonline.com/doi/abs/10.1300/J099v02n04_03#.UqnN9OKQO8E
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Publication Type:
i
Report
Author(s):Suguira, Kiyoyuki; Inokuma, Chie; Imaizumi, Nobuyuki; Cho, Chol
Author Affiliation:Suguira and Inokuma: Research Associate; Imaizumi and Cho: Senior Research Scientist, Japan Turfgrass, Inc.
Title:Generation of herbicide resistant creeping bentgrass (Agrostis palustris Huds.) plants by electroporation-mediated direct gene transfer into protoplasts
Source:Journal of Turfgrass Management. Vol. 2, No. 4, 1999, p. 35-41.
# of Pages:7
Publishing Information:Binghamton, NY: Food Products Press (Haworth Press)
Keywords:TIC Keywords: Herbicide resistance; Agrostis stolonifera; Electroporation; Transgenes; Calli; Genetic transformation; Glufosinate
Abstract/Contents:"Phosphinothricin N-acetyl transferase gene (pat) was introduced into creeping bentgrass (Agrostis palustris Huds.) protoplasts by electroporation-mediated direct gene transfer. Bialaphos resistant calli were yielded through selection with a concentration of 5 mg/l and several plantlets were generated from the calli. The polymerase chain reaction and Southern hybridization analyses of randomly chosen plants indicated that the transgene was integrated into their genomes. Application of herbicide to transgenic creeping bentgrass plants showed no necrosis, demonstrating the expression and functional activity of pat gene in transgenic plants."
Language:English
References:8
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Suguira, K., C. Inokuma, N. Imaizumi, and C. Cho. 1999. Generation of herbicide resistant creeping bentgrass (Agrostis palustris Huds.) plants by electroporation-mediated direct gene transfer into protoplasts. J. Turfgrass Manage. 2(4):p. 35-41.
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Web URL(s):
http://www.tandfonline.com/doi/abs/10.1300/J099v02n04_03#.UqnN9OKQO8E
    Last checked: Item not verified
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
    Notes: Abstract and Guide page only
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MSU catalog number: SB 433 .J68
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