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Web URL(s): | http://www.tandfonline.com/doi/abs/10.1300/J099v02n04_03#.UqnN9OKQO8E Last checked: Item not verified Requires: PDF Reader Access conditions: Item is within a limited-access website Notes: Abstract and Guide page only |
Publication Type:
| Report |
Author(s): | Suguira, Kiyoyuki;
Inokuma, Chie;
Imaizumi, Nobuyuki;
Cho, Chol |
Author Affiliation: | Suguira and Inokuma: Research Associate; Imaizumi and Cho: Senior Research Scientist, Japan Turfgrass, Inc. |
Title: | Generation of herbicide resistant creeping bentgrass (Agrostis palustris Huds.) plants by electroporation-mediated direct gene transfer into protoplasts |
Source: | Journal of Turfgrass Management. Vol. 2, No. 4, 1999, p. 35-41. |
Publishing Information: | Binghamton, NY: Food Products Press (Haworth Press) |
# of Pages: | 7 |
Keywords: | TIC Keywords: Herbicide resistance; Agrostis stolonifera; Electroporation; Transgenes; Calli; Genetic transformation; Glufosinate
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Abstract/Contents: | "Phosphinothricin N-acetyl transferase gene (pat) was introduced into creeping bentgrass (Agrostis palustris Huds.) protoplasts by electroporation-mediated direct gene transfer. Bialaphos resistant calli were yielded through selection with a concentration of 5 mg/l and several plantlets were generated from the calli. The polymerase chain reaction and Southern hybridization analyses of randomly chosen plants indicated that the transgene was integrated into their genomes. Application of herbicide to transgenic creeping bentgrass plants showed no necrosis, demonstrating the expression and functional activity of pat gene in transgenic plants." |
Language: | English |
References: | 8 |
Note: | Pictures, b/w Figures Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Suguira, K., C. Inokuma, N. Imaizumi, and C. Cho. 1999. Generation of herbicide resistant creeping bentgrass (Agrostis palustris Huds.) plants by electroporation-mediated direct gene transfer into protoplasts. J. Turfgrass Manage. 2(4):p. 35-41. |
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| Web URL(s): http://www.tandfonline.com/doi/abs/10.1300/J099v02n04_03#.UqnN9OKQO8E Last checked: Item not verified Requires: PDF Reader Access conditions: Item is within a limited-access website Notes: Abstract and Guide page only |
| MSU catalog number: SB 433 .J68 |
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