Full TGIF Record # 63042
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Web URL(s):https://dl.sciencesocieties.org/publications/cs/pdfs/39/6/1676
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https://dl.sciencesocieties.org/publications/cs/articles/39/6/1676
    Last checked: 12/09/2016
    Access conditions: Item is within a limited-access website
Publication Type:
i
Refereed
Author(s):Chen, C.; Sleper, D. A.
Author Affiliation:Chen: Harvard Institute of Human Genetics, Harvard Medical School Boston, MA; and Sleper: Department of Agronomy, University of Missouri-Columbia, Columbia, MO
Title:FISH and RFLP marker-assisted introgression of Festuca mairei chromosomes into Lolium perenne
Section:Crop breeding, genetics & cytology
Other records with the "Crop breeding, genetics & cytology" Section
Source:Crop Science. Vol. 39, No. 6, November/December 1999, p. 1676-1679.
Publishing Information:Madison, WI: Crop Science Society of America
# of Pages:4
Related Web URL:https://dl.sciencesocieties.org/publications/cs/abstracts/39/6/CS0390061676
    Last checked: 12/09/2016
    Notes: Abstract only
Keywords:TIC Keywords: Lolium perenne; Festuca arundinacea; Festuca; Genetic engineering; Cross breeding; Hybrids; Restriction fragment length polymorphism; Genetic markers; Clones; Backcrossing; DNA; Chromosomes; Quality; Drought resistance; Hybridization
Abstract/Contents:"Plant breeders and geneticists have attempted for several decades to combine perennial ryegrass (Lolium perenne L.) with fescue (Festuca spp.) to create novel forage grasses containing both high forage quality and good drought tolerance. Difficulty in selecting true hybrids with alien chromatin or chromosome addition-substitution has been a major barrier in Festuca x Lolium breeding programs. In this investigation, fluorescence in situ hybridization (FISH) and restriction fragment length polymorphism (RFLP) markers were used to monitor transfer of Festuca mairei St. Yves chromosomes into L. perenne through intergeneric hybrids. Among 64 hybrid plants of the BC₁G₁ generation (intercrossed progeny of first backcross to L. perenne), chromosome addition and substitution of F. mairei were identified by FISH using total genomic DNA of F. mairei as a probe. Forty-two clones from a Pst I-genomic DNA library of F. arundinacea Schreb. were used to screen for the presence of F. mairei DNA in the hybrid plants. These RFLP probes rapidly identified presence of the F. mairei genome in F₁, F₂, BC₁, but not in BC₂ plants. In contrast, genomic FISH on meiotic cells effectively detected any F. mairei chromosomes as well as chromosomal pairing relationships in any hybrid. By monitoring and selectively introducing F. mairei chromosomes into ryegrass, these molecular markers may accelerate the Festuca x Lolium breeding for improvement of ryegrass."
Language:English
References:12
See Also:Other items relating to: Breeding for Drought
Note:Figures
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Chen, C., and D. A. Sleper. 1999. FISH and RFLP marker-assisted introgression of Festuca mairei chromosomes into Lolium perenne. Crop Sci. 39(6):p. 1676-1679.
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Web URL(s):
https://dl.sciencesocieties.org/publications/cs/pdfs/39/6/1676
    Last checked: 12/09/2016
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
https://dl.sciencesocieties.org/publications/cs/articles/39/6/1676
    Last checked: 12/09/2016
    Access conditions: Item is within a limited-access website
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MSU catalog number: SB 183 .C7
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