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Web URL(s): | https://dl.sciencesocieties.org/publications/cs/pdfs/39/6/1676 Last checked: 12/09/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/articles/39/6/1676 Last checked: 12/09/2016 Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Chen, C.;
Sleper, D. A. |
Author Affiliation: | Chen: Harvard Institute of Human Genetics, Harvard Medical School Boston, MA; and Sleper: Department of Agronomy, University of Missouri-Columbia, Columbia, MO |
Title: | FISH and RFLP marker-assisted introgression of Festuca mairei chromosomes into Lolium perenne |
Section: | Crop breeding, genetics & cytology Other records with the "Crop breeding, genetics & cytology" Section
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Source: | Crop Science. Vol. 39, No. 6, November/December 1999, p. 1676-1679. |
Publishing Information: | Madison, WI: Crop Science Society of America |
# of Pages: | 4 |
Related Web URL: | https://dl.sciencesocieties.org/publications/cs/abstracts/39/6/CS0390061676 Last checked: 12/09/2016 Notes: Abstract only |
Keywords: | TIC Keywords: Lolium perenne; Festuca arundinacea; Festuca; Genetic engineering; Cross breeding; Hybrids; Restriction fragment length polymorphism; Genetic markers; Clones; Backcrossing; DNA; Chromosomes; Quality; Drought resistance; Hybridization
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Abstract/Contents: | "Plant breeders and geneticists have attempted for several decades to combine perennial ryegrass (Lolium perenne L.) with fescue (Festuca spp.) to create novel forage grasses containing both high forage quality and good drought tolerance. Difficulty in selecting true hybrids with alien chromatin or chromosome addition-substitution has been a major barrier in Festuca x Lolium breeding programs. In this investigation, fluorescence in situ hybridization (FISH) and restriction fragment length polymorphism (RFLP) markers were used to monitor transfer of Festuca mairei St. Yves chromosomes into L. perenne through intergeneric hybrids. Among 64 hybrid plants of the BCâGâ generation (intercrossed progeny of first backcross to L. perenne), chromosome addition and substitution of F. mairei were identified by FISH using total genomic DNA of F. mairei as a probe. Forty-two clones from a Pst I-genomic DNA library of F. arundinacea Schreb. were used to screen for the presence of F. mairei DNA in the hybrid plants. These RFLP probes rapidly identified presence of the F. mairei genome in Fâ, Fâ, BCâ, but not in BCâ plants. In contrast, genomic FISH on meiotic cells effectively detected any F. mairei chromosomes as well as chromosomal pairing relationships in any hybrid. By monitoring and selectively introducing F. mairei chromosomes into ryegrass, these molecular markers may accelerate the Festuca x Lolium breeding for improvement of ryegrass." |
Language: | English |
References: | 12 |
See Also: | Other items relating to: Breeding for Drought |
Note: | Figures Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Chen, C., and D. A. Sleper. 1999. FISH and RFLP marker-assisted introgression of Festuca mairei chromosomes into Lolium perenne. Crop Sci. 39(6):p. 1676-1679. |
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| Web URL(s): https://dl.sciencesocieties.org/publications/cs/pdfs/39/6/1676 Last checked: 12/09/2016 Requires: PDF Reader Access conditions: Item is within a limited-access website https://dl.sciencesocieties.org/publications/cs/articles/39/6/1676 Last checked: 12/09/2016 Access conditions: Item is within a limited-access website |
| MSU catalog number: SB 183 .C7 |
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