Full TGIF Record # 63104
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Jiang, Z.; Hull, R. J.
Author Affiliation:University of Rhode Island
Title:In vivo assay of nitrate and nitrite reductase activities in Kentucky bluegrass
Section:Turfgrass science
Other records with the "Turfgrass science" Section
Meeting Info.:Salt Lake City, UT: October 31 - November 4, 1999
Source:1999 Annual Meeting Abstracts [ASA/CSSA/SSSA]. Vol. 91, 1999, p. 128.
Publishing Information:[Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America
# of Pages:1
Keywords:TIC Keywords: Nitrates; Nitrites; Nitrite reductase; Nitrate reductase; Poa pratensis; Enzyme activity; Enzymes; Metabolism; Nitrogen
Abstract/Contents:"In vivo assays for nitrate reductase activity (NRA) have been used extensively in studies of plant N metabolism, whereas in vivo assays for nitrite reductase activity (NiRA) have not often been employed. The objective of this study was to optimize assay conditions for in vivo NRA and NiRA in Kentucky bluegrass (Poa pratensis L.) leaves. Maximum NRA was obtained by using a medium containing 100 mM potassium phosphate buffer (pH 7.5), 390 mM 2-propanol and 50 mM potassium nitrate. Lower pH and higher concentrations of propanol inhibited NRA. Dark and anaerobic conditions were essential for assays of NRA. Under illumination, nitrite was absorbed by leaf tissues and reduced by nitrite reductase to ammonium, which accumulated in the presence of methionine sulfoximine. Based on this observation, an in vivo NiRA assay was developed. The use of these methods for studying turfgrass N metabolism will be discussed."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jiang, Z., and R. J. Hull. 1999. In vivo assay of nitrate and nitrite reductase activities in Kentucky bluegrass. Annu. Meet. Abstr. 91:p. 128.
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