Full TGIF Record # 63270
Item 1 of 1
Publication Type:
i
Refereed
Author(s):Kelly, Steven T.; Coats, G. Euel; Luthe, Dawn S.
Author Affiliation:Kelly: Senior Research Assistant, and Coats: Professor, Department of Plant and Soil Sciences, and Luthe: Professor, Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS
Title:Mode of resistance of triazine-resistant annual bluegrass (Poa annua)
Section:Research
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Source:Weed Technology. Vol. 13, No. 4, October-December 1999, p. 747-752.
Publishing Information:Champaign, IL: Weed Science Society of America.
# of Pages:6
Keywords:TIC Keywords: Poa annua; Anilazine; Biotypes; Mechanism of resistance; Herbicide resistance; Mode of action; Clipping weight; DNA; Genetics; DNA amplification; Simazine; Diuron
Abstract/Contents:"Two previously identified triazine-resistant annual bluegrass (Poa annua) biotypes in Mississippi were evaluated to determine the level as well as the mode of resistance compared to a triazine-susceptible biotype. Annual bluegrass seeds were collected at two sites (Meridian and Tupelo, MS) from field plots previously treated with 11.2 kg ai/ha simazine. Seeds were planted on agar containing simazine (0, 0.001, 0.01, 0.1, 1, 10, or 100 mM) or diuron (0, 0.01, 0.1, 1, 10, 100, or 1,000 μM), placed in the growth chamber with an 8 hour daylength and alternating temperatures of 15 and 20 C, and allowed to grow for 3 wk, after which fresh weight was determined. Data were subjected to regression analyses and the amount of simazine or diuron required to reduce fresh weight to 50% of the untreated plants was determined. These data indicated agreater than 1,000-fold level of resistance to simazine with either biotype. Diuron concentrations of 5.0, 3.0, and 3.2 μM were required to reduce fresh weight to 50% of the untreated for the triazine-susceptible, Meridian, and Tupelo biotypes, respectively. Sequencing a portion of the chloroplast psbA gene revealed a serine 264 to glycine mutation, which reduced the affinity of the triazine herbicides for the Q^D[B-binding niche on the D1 protein in photosystem II."
Language:English
References:19
Note:Tables
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kelly, S. T., G. E. Coats, and D. S. Luthe. 1999. Mode of resistance of triazine-resistant annual bluegrass (Poa annua). Weed Technol. 13(4):p. 747-752.
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