Full TGIF Record # 63408
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Author(s):Okora, J. O.; Watson, C. E.; Gourley, L. M.; Keith, B. C.; Vaughan, C. E.
Author Affiliation:Mississippi Agricultural and Forestry Experiment Station, Mississippi State, MS
Title:Comparison of botanical characteristics and seedling root fluorescence for distinguishing Italian and perennial ryegrass
Source:Seed Science and Technology. Vol. 27, No. 2, 1999, p. 721-730.
# of Pages:10
Publishing Information:International Seed Testing Association
Keywords:TIC Keywords: Lolium multiflorum; Lolium perenne; Identification; Seed testing; Methodology; Tissue testing; Phenotypes; Comparisons; Growth habit; Color; Leaf width; Leaf blade length; Height; Spikelets; Florets; Fluorescence microscopy
Abstract/Contents:"The seedling root fluorescence test is used by many seed regulatory agencies to distinguish between Italian (Lolium multiflorum Lam.) and perennial (L. perenne L.) ryegrass seeds. The objectives of this study were to examine phenotypic differences between fluorescent and non-fluorescent Italian and perennial ryegrass plants, and to compare classification of Italian and perennial ryegrass using either seedling root fluorescence or botanical characteristics. Fourteen populations were generated by dividing four Italian and three perennial ryegrass cultivars into fluorescent and non-fluoresent populations. Populations were evaluated for leaf color, growth habit, leaf vernation (rolled or folded), leaf width, and leaf length in the greenhouse and field. Plant height , spike length, spikelets spikeā»Ā¹ and florets spikeletā»Ā¹ were also measured in the field. Populations differed significantly for all characteristics. Population means and generalized squared distances between populations for botanical characteristics were not significantly different for fluorescent and non-fluorescent populations within a given cultivar. Seedling root fluorescence was not linked to species or botanical characteristics. Stepwise discriminant analysis indicated that the rolled leaves of L. multiflorum discriminated it from L. perenne which has folded leaves. Discrimination based on leaf veneration alone was identical to that using all botanical characteristics and root fluorescence. A test based on leaf vernation should offer more reliable results than the seedling root flourescence test for distinguishing Italian and perennial ryegrass."
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Okora, J. O., C. E. Watson, L. M. Gourley, B. C. Keith, and C. E. Vaughan. 1999. Comparison of botanical characteristics and seedling root fluorescence for distinguishing Italian and perennial ryegrass. Seed Sci. Technol. 27(2):p. 721-730.
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