Full TGIF Record # 63415
Item 1 of 1
Publication Type:
Content Type:Abstract or Summary only
Author(s):Warnke, S. E.; Barker, R. E.
Author Affiliation:Warnke: Oregon State University; and Barker: USDA-ARS
Title:DNA based tests for discriminating ryegrass types
Section:Turfgrass science
Other records with the "Turfgrass science" Section
Meeting Info.:Salt Lake City, UT: October 31 - November 4, 1999
Source:1999 Annual Meeting Abstracts [ASA/CSSA/SSSA]. Vol. 91, 1999, p. 133.
# of Pages:1
Publishing Information:[Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America
Keywords:TIC Keywords: DNA; Lolium perenne; Lolium multiflorum; Random amplified polymorphic DNA markers; Genetic markers; Identification; Techniques; Comparisons
Abstract/Contents:"The seedling root fluorescence (SRF) test has been used for seventy years to distinguish annual ryegrass (Lolium multiflorum Lam.) from perennial ryegrass (L. perenne L.). The SRF test does serve as a general indicator of annual ryegrass contamination of perennial ryegrass seed lots. The SRF character, however, is a poor indicator of genetic contamination between these two interfertile species. DNA testing would provide a fast, and accurate alternative to the SRF test. Screening of over 700 RAPD primers in a backcross population using Bulked Segregant Analysis has yielded no useful linkages with the SRF character. Esterase isozyme analysis indicates that the reported esterase difference between annual and perennial ryegrass may be bue to tissue specific expression of the esterase locus. A comparative genetics approach using markers linked to genes influencing vernalization requirement in barley may provide useful information in the development of a replacement test for SRF."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Warnke, S. E., and R. E. Barker. 1999. DNA based tests for discriminating ryegrass types. Annu. Meet. Abstr. 91:p. 133.
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