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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Kobayashi, Donald;
Holtman, M. Andrew. |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University |
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| Title: | Characterization of a chitinase gene from Stenotrophomonas maltophilia 34S1, and establishment of its role in biological control of summer patch |
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| Section: | Plenary presentations
Other records with the "Plenary presentations" Section
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| Meeting Info.: | January 16-17, 1997: Cook College |
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| Source: | Proceedings of the Sixth Annual Rutgers TurfgrassSymposium. 1997, p. 18. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Disease control; Genes; Stenotrophomonas maltophilia; Chitinase; Summer patch; Biological control
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| Abstract/Contents: | "Stenotrophomonas maltophilia effectively suppresses summer patch disease on Kentucky bluegrass. The suppressive ability is thought to result in part from production of the enzyme chitinase. A single chitinase gene was cloned from a genomic library of Sm34S1. This chitinase gene, designated chiA, resides on a 3.0 kb XhoI-SacI fragment. Sequence analysis suggests an open reading frame of 2.0 kb and a translational product of 91 kDa. Confirmation of the predicted product was determined by heterologous expression studies of chiA in Burkholderia cepacia, resulting in a protein of approximately 88 kDa. Characterization of cell-free culture filtrates of Sm34S1 indicate the presence of a major protein band of 51.1 kDa. Corresponding native polyacrylamide gels associate this major protein band with chitinase activity. Site-directed marker exchange with the transposon Tn5 produced a chiA⁻ mutant. The resulting mutant, C5, no longer displayed chitinolytic activity, and no longer produced the 51.1 kDa protein in culture filtrates. When the cloned chitinase gene was mobilized into C5, chitinase activity was restored to the mutant, along with production of the 51.1 kDa protein in culture filtrates. These results indicate that the 51.1 kDa protien is the major enzyme responsible for observed chitinolytic activity in Sm34S1, and this activity is conferred by the cloned chiA gene. The differential size between the mature protien and the cellular protein is likely due to posttranslational processing in Sm34S1. Comparisons of the chiA⁻ mutant C5 with Sm34S1 indicated that summer patch disease suppression was significantly reduced in bioassays, but rhizosphere and soil populations were not significantly compromised. These results indicate that chitinase production by Sm34S1 is important in the control of summer patch, and provides the basis for further studies directed at enhancing biocontrol performances in field situations." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kobayashi, D., and M. A. Holtman. 1997. Characterization of a chitinase gene from Stenotrophomonas maltophilia 34S1, and establishment of its role in biological control of summer patch. Proc. Annu. Rutgers Turfgrass Symp. p. 18. |
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