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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Holtman, M. Andrew;
Kobayashi, Donald Y. |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University |
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| Title: | Phylogenetic analysis of biocontrol strain N4-7, isolated from a New Jersey golf course |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | January 16-17, 1997: Cook College |
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| Source: | Proceedings of the Sixth Annual Rutgers TurfgrassSymposium. 1997, p. 31. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Phylogeny; Organic acids; Biological control; Xanthomonas
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| Abstract/Contents: | "Phylogenetic position of strain N4-7 was studied on the basis of morphological, biochemical, 16s rDNA sequence and serological data. Isolate N4-7 is gram-negative, non-motile with possible fimbriae, aerobic, rod-shaped (0.3 x 1.5 mm) and in culture, is yellow and smooth with entire margin. A yellow-brown pigment is produced which peaks at 289 nm (xanthomonadins peak at 445 nm). Various diagnostic organic acids and sugars are utilized by N4-7. Respectively, several of these include: citric, aconitic, a keto-butyric, a keto-glutaric and b-hydroxybutyric acid as well as lactose, maltose, cellobiose, fructose, mannose and trehalose. The isolate is oxidase and catalase positive, arginine dihydrolase negative and liquifies gelatin. Furthermore, N4-7 is likely non-phytopathogenic as determined by HR tests on Nicotiana. 16s rDNA sequence analysis reveals that N4-7 is closely related to Xanthomonas sp., Stenotrophomonas sp., and Xylella fastidiosa while being nearly identical to a set of unclassified hydrothermal vent eubacteria. Fatty acid profiles consisting of a large percentage of unsaturated 15:0 iso, saturated 16:1 and 17:1 iso fail to match N4-7 as a species to any of the above mentioned genus but suggest a relatedness to Xanthomonas. Serological tests using polyclonal antibodies to N4-7 show cross-reactivity to Xylella fastidiosa and to Stenotrophomonas maltophilia but not to Xanthomonas sp. Based upon the above evidence, isolate N4-7 is best grouped separately as a new genus." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Holtman, M. A., and D. Y. Kobayashi. 1997. Phylogenetic analysis of biocontrol strain N4-7, isolated from a New Jersey golf course. Proc. Annu. Rutgers Turfgrass Symp. p. 31. |
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MSU catalog number: SB 433 .R88 |
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