Full TGIF Record # 63799
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Redwine, S. M.; Baird, J. H.; Sticklen, M.
Author Affiliation:Michigan State University
Title:Mannitol accumulation in transgenic turfgrass
Section:Turfgrass science
Other records with the "Turfgrass science" Section
Meeting Info.:Salt Lake City, UT: October 31 - November 4, 1999
Source:1999 Annual Meeting Abstracts [ASA/CSSA/SSSA]. Vol. 91, 1999, p. 138.
Publishing Information:[Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America
# of Pages:1
Keywords:TIC Keywords: Agrostis stolonifera; Transgenic turfgrasses; Genetic transformation; Genes; Genetics; Salt tolerance; Drought resistance
Cultivar Names:Penncross
Abstract/Contents:"Creeping bentgrass (Agrostis palustris Huds., cv. Penncross) was genetically transformed by biolistic insertion of the mannitol 1-phosphate dehydrogenase [mtlD] gene. The mtlD gene is reported to aid in drought and salinity tolerance via osmoregulation through the production of mannitol. Mannitol accumulation in tissues of regenerated clones and non-transformed Penncross was evaluated under salt and drought stress conditions. Salt stress was induced for 4 days by irrigation with 100 mM NaCl, followed by irrigation with tap water for 3 days. Drought stress was induced by subjecting a separate set of plants to 10% soil moisture before a 3 day recovery period. Carbohydrates were extracted three times by heating whole plants to 70 C in 80% ethanol for 30 minutes. Extracts were dried then rehydrated using millipore water. Mannitol content was measured using high-performance liquid chromatography (HLPC) using a Shodex sugar SC1101 column."
See Also:Other items relating to: SALTT
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Redwine, S. M., J. H. Baird, and M. Sticklen. 1999. Mannitol accumulation in transgenic turfgrass. Annu. Meet. Abstr. 91:p. 138.
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MSU catalog number: S 1 .A58
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