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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Redwine, S. M.;
Baird, J. H.;
Sticklen, M. |
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| Author Affiliation: | Michigan State University |
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| Title: | Mannitol accumulation in transgenic turfgrass |
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| Section: | Turfgrass science
Other records with the "Turfgrass science" Section
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| Meeting Info.: | Salt Lake City, UT: October 31 - November 4, 1999 |
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| Source: | 1999 Annual Meeting Abstracts [ASA/CSSA/SSSA]. Vol. 91, 1999, p. 138. |
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| # of Pages: | 1 |
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| Publishing Information: | [Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Transgenic turfgrasses; Genetic transformation; Genes; Genetics; Salt tolerance; Drought resistance
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| Cultivar Names: | Penncross |
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| Abstract/Contents: | "Creeping bentgrass (Agrostis palustris Huds., cv. Penncross) was genetically transformed by biolistic insertion of the mannitol 1-phosphate dehydrogenase [mtlD] gene. The mtlD gene is reported to aid in drought and salinity tolerance via osmoregulation through the production of mannitol. Mannitol accumulation in tissues of regenerated clones and non-transformed Penncross was evaluated under salt and drought stress conditions. Salt stress was induced for 4 days by irrigation with 100 mM NaCl, followed by irrigation with tap water for 3 days. Drought stress was induced by subjecting a separate set of plants to 10% soil moisture before a 3 day recovery period. Carbohydrates were extracted three times by heating whole plants to 70 C in 80% ethanol for 30 minutes. Extracts were dried then rehydrated using millipore water. Mannitol content was measured using high-performance liquid chromatography (HLPC) using a Shodex sugar SC1101 column." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| See Also: | Other items relating to: SALTT |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Redwine, S. M., J. H. Baird, and M. Sticklen. 1999. Mannitol accumulation in transgenic turfgrass. Annu. Meet. Abstr. 91:p. 138. |
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MSU catalog number: S 1 .A58 |
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