Full TGIF Record # 63880
Item 1 of 1
Publication Type:
Content Type:Abstract or Summary only
Author(s):Bonos, S. A.; Plumley, K. A.; Meyer, W. A.
Author Affiliation:New Jersey Agricultural Experiment Station
Title:The use of laser flow cytometry for species determination in Agrostis
Section:Turfgrass science
Other records with the "Turfgrass science" Section
Meeting Info.:Salt Lake City, UT: October 31 - November 4, 1999
Source:1999 Annual Meeting Abstracts [ASA/CSSA/SSSA]. Vol. 91, 1999, p. 140.
# of Pages:1
Publishing Information:[Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America
Keywords:TIC Keywords: Laser flow cytometry; Taxonomy; Classification; Agrostis; Identification; Ploidy; Morphology; Agrostis canina; Agrostis stolonifera; Agrostis tenuis; Agrostis castellana; Agrostis alba; Techniques; DNA; Chromosome number
Abstract/Contents:"The taxonomic classification of the genus Agrostis based upon morphological and anatomical characters is difficult and complicated by the misapplication of names. The objective of this study was to: 1) evaluate the use of laser flow cytometry as a quick, reliable tool to aid in species determination of Agrostis, based on ploidy level and 2) identify which morphological characters were associated with ploidy level. The 6 Agrostis species evaluated were A. canina L. subspecies canina (2n=2x=14), A. canina L. subspecies montana (Hartm.) Hartm. (2n=4x=28), A. palustris Huds. (2n=4x=28), A. tenius Sibth. (A. capillaris L.) (2n=4x=28), A. castellana Boiss. & Reut. (2n=6x=42), and A. alba L. (2n=6x=42). Plant material was prepared for flow cytometry analysis following a modified technique reported by Huff and Palazzo (1998). Significant differences in 2C DNA were found between species (P < 0.01). Reported chromosome numbers were positively correlated with observed 2C DNA content (r = 0.94, P < 0.01). These results indicate that laser flow cytometry is a quick, reliable tool to distinguish certain species of Agrostis."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bonos, S. A., K. A. Plumley, and W. A. Meyer. 1999. The use of laser flow cytometry for species determination in Agrostis. Annu. Meet. Abstr. 91:p. 140.
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