Publication Type:
i
Report Content Type: Abstract or Summary only Author(s): Bonos, S. A. ;
Plumley, K. A. ;
Meyer, W. A. Author Affiliation: New Jersey Agricultural Experiment Station Title: The use of laser flow cytometry for species determination in Agrostis Section: Turfgrass scienceOther records with the "Turfgrass science" Section Meeting Info.: Salt Lake City, UT: October 31 - November 4, 1999 Source: 1999 Annual Meeting Abstracts [ASA/CSSA/SSSA]. Vol. 91, 1999, p. 140.# of Pages: 1 Publishing Information: [Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America Keywords: TIC Keywords: Laser flow cytometry ; Taxonomy ; Classification ; Agrostis ; Identification ; Ploidy ; Morphology ; Agrostis canina ; Agrostis stolonifera ; Agrostis tenuis ; Agrostis castellana ; Agrostis alba ; Techniques ; DNA ; Chromosome number Abstract/Contents: "The taxonomic classification of the genus Agrostis based upon morphological and anatomical characters is difficult and complicated by the misapplication of names. The objective of this study was to: 1) evaluate the use of laser flow cytometry as a quick, reliable tool to aid in species determination of Agrostis , based on ploidy level and 2) identify which morphological characters were associated with ploidy level. The 6 Agrostis species evaluated were A. canina L. subspecies canina (2n=2x=14), A. canina L. subspecies montana (Hartm.) Hartm. (2n=4x=28), A. palustris Huds. (2n=4x=28), A. tenius Sibth. (A. capillaris L.) (2n=4x=28), A. castellana Boiss. & Reut. (2n=6x=42), and A. alba L. (2n=6x=42). Plant material was prepared for flow cytometry analysis following a modified technique reported by Huff and Palazzo (1998). Significant differences in 2C DNA were found between species (P < 0.01). Reported chromosome numbers were positively correlated with observed 2C DNA content (r = 0.94, P < 0.01). These results indicate that laser flow cytometry is a quick, reliable tool to distinguish certain species of Agrostis ." Language: English References: 0 Note: This item is an abstract only! ASA/CSSA/SSSA Citation (Crop Science -Like - may be incomplete ): Agrostis . Annu. Meet. Abstr. 91:p. 140.Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=63880 If there are problems with this record, send us feedback about record 63880 . Choices for finding the above item : S 1 .A58 Request through your local library's inter-library loan service (bring or send a copy of this TGIF record)
