| |
| Publication Type:
| Report |
|---|
| Content Type: | Abstract or Summary only |
|---|
| Author(s): | Bonos, S. A.;
Plumley, K. A.;
Meyer, W. A. |
|---|
| Author Affiliation: | New Jersey Agricultural Experiment Station |
|---|
| Title: | The use of laser flow cytometry for species determination in Agrostis |
|---|
| Section: | Turfgrass science
Other records with the "Turfgrass science" Section
|
|---|
| Meeting Info.: | Salt Lake City, UT: October 31 - November 4, 1999 |
|---|
| Source: | 1999 Annual Meeting Abstracts [ASA/CSSA/SSSA]. Vol. 91, 1999, p. 140. |
|---|
| Publishing Information: | [Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America |
|---|
| # of Pages: | 1 |
|---|
| Keywords: | TIC Keywords: Laser flow cytometry; Taxonomy; Classification; Agrostis; Identification; Ploidy; Morphology; Agrostis canina; Agrostis stolonifera; Agrostis tenuis; Agrostis castellana; Agrostis alba; Techniques; DNA; Chromosome number
|
|---|
| Abstract/Contents: | "The taxonomic classification of the genus Agrostis based upon morphological and anatomical characters is difficult and complicated by the misapplication of names. The objective of this study was to: 1) evaluate the use of laser flow cytometry as a quick, reliable tool to aid in species determination of Agrostis, based on ploidy level and 2) identify which morphological characters were associated with ploidy level. The 6 Agrostis species evaluated were A. canina L. subspecies canina (2n=2x=14), A. canina L. subspecies montana (Hartm.) Hartm. (2n=4x=28), A. palustris Huds. (2n=4x=28), A. tenius Sibth. (A. capillaris L.) (2n=4x=28), A. castellana Boiss. & Reut. (2n=6x=42), and A. alba L. (2n=6x=42). Plant material was prepared for flow cytometry analysis following a modified technique reported by Huff and Palazzo (1998). Significant differences in 2C DNA were found between species (P < 0.01). Reported chromosome numbers were positively correlated with observed 2C DNA content (r = 0.94, P < 0.01). These results indicate that laser flow cytometry is a quick, reliable tool to distinguish certain species of Agrostis." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only! |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bonos, S. A., K. A. Plumley, and W. A. Meyer. 1999. The use of laser flow cytometry for species determination in Agrostis. Annu. Meet. Abstr. 91:p. 140. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=63880 |
| If there are problems with this record, send us feedback about record 63880. |
| Choices for finding the above item: |
|---|
| 
MSU catalog number: S 1 .A58 |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
