Full TGIF Record # 65526
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Publication Type:
Author(s):Blanche, F. C. B.; Krans, J. V.
Author Affiliation:Blanche: Research Assistant; Krans: Professor of Agronomy, Mississippi State University
Title:Selection for heat tolerance in creeping bentgrass using tissue culture
Meeting Info.:July 16, 1991
Source:Mississippi State Turfgrass Field Day. 1991, p. 20-21.
# of Pages:2
Publishing Information:[Mississippi State: Mississippi State University]
Keywords:TIC Keywords: Agrostis stolonifera; Calli; Root growth; Tissues; Drought; Heat resistance
Abstract/Contents:The study was conducted to 1) evaluate the effect of heat on callus growth and plantlet regeneration of two creeping bentgrass variants and Penncross, and 2) to evaluate schemes in selecting for heat tolerance. The two variants consisted for MSCB 6 and MSCB 8. MSCB 6 was originally a line of Penncross previously selected in tissue culture at 35°C while MSCB 8 was an ecotype that had been growing at the Plant Science Farm, MSU. Both variants have been field-evaluated at extreme high temperature and drought conditions and were found to have outstanding performance. Different light periods and temperature variants were placed on the clusters. Callus growth was evaluated by obtaining fresh weights at 10, 20 and 30 days incubation. Plantlet formation and root growth were determined after 30, 40 and 50 days. Results have shown that MSVB 6 had significantly highest mean values for callus growth at all schemes after 10, 20, and 30 days incubation. MSCB 8 had slightly lower callus weights than Penncross at 25±2°C but showed higher weights than Penncross at high temperature. Callus growth of both variants were higher at 25±2°C (or room temp.) than at 40±3°C throughout the duration of incubation. Other findings include the specific schemes and the effect on callus growth.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Blanche, F. C. B., and J. V. Krans. 1991. Selection for heat tolerance in creeping bentgrass using tissue culture. Miss. State Turfgrass Field Day. p. 20-21.
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MSU catalog number: SB 433.16 .M7 M57
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