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DOI: | 10.21273/HORTSCI.35.3.452B |
Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/35/3/article-p452B.xml?rskey=VGIqoR Last checked: 11/14/2019 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Hughes, Harrison;
Towill, Leigh |
Author Affiliation: | Hughes: Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO; Towill: National Seed Storage Laboratory, USDA, ARS, Fort Collins, CO |
Title: | In vitro establishment and growth of bermudagrass, buffalograss, saltgrass, and zoysiagrass |
Section: | Poster session 20: Propagation/tissue culture Other records with the "Poster session 20: Propagation/tissue culture" Section
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Meeting Info.: | 97th International Conference of the American Society for Horticultural Science, 23-26 July, 2000, Lake Buena Vista, FL |
Source: | HortScience. Vol. 35, No. 3, June 2000, p. 452. |
Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
# of Pages: | 1 |
Keywords: | TIC Keywords: In vitro; Establishment; Growth; Propagation; Methodology; Comparisons; Cynodon; Bouteloua dactyloides; Distichlis spicata; Zoysia; Growth regulators; Micropropagation; Techniques; Tissue culture; Genetics
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Abstract/Contents: | "There are turfgrasses species that are clonally propagated; notably bermudagrass, buffalograss, and zoysiagrass. Some of the early cultivars of these species are no longer widely grown, and may eventually be lost if not preserved. In order to facilitate studies on the long-term cryopreservation of these species and specific lines of saltgrass, it is necessary to develop suitable micropropagation procedures. We have developed protocol for the isolation and establishment of clean cultures in vitro for all four species. A 1/2-strength MS basal medium with Nitsch & Nitsch vitamins, 5 mg/L of thiamine, 2 mg/L of glycine, 30 g of sucrose, 7 g of agar with varying growth regulators has been used. Explant materials are prewashed in the greenhouse prior to a 15-to 30-min soapy wash in the laboratory. After a 30- to 60-min rinse in running water, nodal sections are surface-disinfected in 10% bleach with Tween 20 for 15 min, followed by three sterile water rinses. This procedure, sometimes with PPM (a properietary antimicrobial compound), results in 50% or greater clean cultures. Rapidly growing nodal sections work best and preferably those not established in soil. We have tested various growth regulator combinations and have found that 10 mg/L of BA results in proliferation of buffalograss and saltgrass. However, proliferation remains relatively slow, requiring 8 to 12 weeks to develop sufficiently for subculture. Although we have succeeded in obtaining clean cultures of bermudagrass and zoysiagrass, proliferation is minimal. Further research is ongoing to develop a proliferative system with these two species." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Hughes, H., and L. Towill. 2000. In vitro establishment and growth of bermudagrass, buffalograss, saltgrass, and zoysiagrass. HortScience. 35(3):p. 452. |
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| DOI: 10.21273/HORTSCI.35.3.452B |
| Web URL(s): https://journals.ashs.org/hortsci/view/journals/hortsci/35/3/article-p452B.xml?rskey=VGIqoR Last checked: 11/14/2019 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 1 .H64 |
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