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| Web URL(s): | https://link.springer.com/article/10.1007/s0012800086
Last checked: 09/27/2017
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Report |
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| Author(s): | MacKenzie, B. A.;
Striley, C. A. F.;
Biagini, R. E.;
Stettler, L. E.;
Hines, C. J. |
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| Author Affiliation: | MacKenzie, Striley, Biagini, and Stettler: Department of Health and Human Services, Public Health Service, Centers of Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Cincinnati, OH; Hines: Department of Health and Human Services, Public Health Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Surveillance Hazards Evaluation and Field Studies, Cincinnati, OH |
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| Title: | Improved rapid analytical method for the urinary determination of 3,5,6 Trichloro-2-Pyridinol, a metabolite of chlorpyrifos |
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| Source: | Bulletin of Environmental Contamination and Toxicology. Vol. 65, No. 1, July 2000, p. 1-7. |
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| Publishing Information: | New York: Springer-Verlag New York |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Chemical applicators; Metabolites; Pesticide safety; Pesticides; Chlorpyrifos; Measurement; Health; Enzyme-linked immunosorbent assay; Gas chromatography; Methodology; Analytical methods; Comparisons; Urine
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| Abstract/Contents: | Presents a study investigating "immunochemical biological monitoring methods for various organophosphate pesticides," particularly chlorpyrifos. "The present investigation was part of a field study of workers who commercially apply chlorpyrifos-containing termiticides. A total of 285 urine samples were submitted from the field, including 28 quality control samples submitted blindly...for immunoassays, a commercially available enzyme linked immunosorbent assay (ELISA) kit primarily designed for the analysis of TCP [3,5,6-trichloro-2-pyridinol] in water, was modified for urinary TCP determinations." Results indicate that the "ELISA kit used for urinary TCP analysis was designed for use in a water matrix, and the urine matrix clearly affects its utility. While sample clean-up procedures might eliminate or reduce the matrix effects, the time required for these steps would negate the advantage in analysis time and cost for the ELISA compared to the instrumental method. Consequently, we chose to use dilution with PBS to minimize matrix effects...the high correlation between methods and the slope of the correlation curve for the ELISA vs. GC-MSD [gas chromatography with mass selective detection] approaching unity, indicates the β-glucuronidase-hydrolyzed ELISA procedure is a viable adjunct to classical instrumental methods for urinary TCP, particularly in screening for occupational exposure to clorpyrifos." |
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| Language: | English |
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| References: | 13 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
MacKenzie, B. A., C. A. F. Striley, R. E. Biagini, L. E. Stettler, and C. J. Hines. 2000. Improved rapid analytical method for the urinary determination of 3,5,6 Trichloro-2-Pyridinol, a metabolite of chlorpyrifos. Bull. Environ. Contam. Toxicol. 65(1):p. 1-7. |
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| Web URL(s):
https://link.springer.com/article/10.1007/s0012800086
Last checked: 09/27/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
| 
MSU catalog number: RA 565 .A1 B8 |
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