| |
| Web URL(s): | https://link.springer.com/article/10.1007%2Fs001220051148
Last checked: 09/27/2017 |
|---|
| Author(s): | Zhang, L. H.;
Ozias-Akins, P.;
Kochert, G.;
Kresovich, S.;
Dean, R.;
Hanna, W. |
|---|
| Author Affiliation: | Zhang: Department of Crop and Soil Sciences; and Ozias-Akins: Horticulture Department, Coastal Plain Experiment Station, University of Georgia, Tifton, GA; Kochert: Botany Department, University of Georgia, Athens, GA; Kresovich and Dean: Plant Germplasm Conservation Unit, USDA-ARS-SAA, Georgia Experiment Station, Griffin, GA; and Hanna: Forage and Turf Unit, USDA-ARS-SAA, Coastal Plain Experiment Station, Tifton, GA |
|---|
| Title: | Differentiation of bermudagrass (Cynodon spp.) genotypes by AFLP analyses |
|---|
| Source: | Theoretical and Applied Genetics. Vol. 98, 1999, p. 895-902. |
|---|
| Publishing Information: | Berlin: Springer |
|---|
| # of Pages: | 8 |
|---|
| Related Web URL: | https://www.springerlink.com/content/bt1h0rud93je7837/?p=2ce3c8ea80b64ca696c5be3438ad1207&pi=5
Last checked: 10/06/2017
Notes: Abstract only |
|---|
| Keywords: | TIC Keywords: Cynodon; Genotypes; Bioassay; Cultivar identification; Differentiation; Amplification fragment length polymorphisms; DNA; DNA amplification; Cynodon dactylon; Cynodon transvaalensis; Methodology; Analytical methods; Genetics; Radioactive labeling
|
|---|
| Abstract/Contents: | "Bermudagrasses (Cynodon spp.) are major turfgrasses for home lawns, public parks, golf courses and sport fields, and are widely adapted to tropical and warmer temperate climates. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass genotypes because the differences between them are often subtle and subject to environmental influence. In this study, a DNA-typing technique, amplified fragment length polymorphism (AFLP), was used to differentiate bermudagrass genotypes and to explore their genetic relationships. Twenty seven bermudagrass cultivars and introductions, mostly from the Coastal Plain Experiment Station in Tifton, Ga., were assayed by the radioactive (³²P) and the fluorescence-labeled AFLP methods. The AFLP technique produced enough polymorphism to differentiate all 27 bermudagrass genotypes, even the closely related ones. An average of 48-74 bands in the 30-600-bp size range was detected by the ³²P-labeled AFLP method. The results indicated that most of the 14 primer combinations tested in this study could be used to distinguish bermudagrass genotypes, and that some single primer-pairs could differentiate all 27 of them. To test the reliability and reproducibility of the AFLP procedure, three DNA isolations (replications) of the 27 bermudagrass genotypes were assayed using five primer pairs. Only 0.6% of the bands were evaluated differently among the three replications. One replication of one genotype (which was most likely a planting contaminant) was grouped in an unexpected cluster using the Unweighted Pair Goup Mean Average (UPGMA) method. A one- or two-band difference in scoring did not change the clustering of genotypes or the replications within genotypes. The 27 genotypes were grouped into three major clusters, many of which were in agreement with known pedigrees. Trees constructed with different primer combinations using ³²P- and fluorescence-labelling formed similar major groupings. The semi-automated fluorescence-based AFLP technique offered significant improvements on fragment sizing and data handling. It was also more accurate for detection and more efficient than the radioactive labelling method. This study shows that the AFLP technique is a reliable tool for differentiating bermudagrass genotypes and for determining genetic relationships among them." |
|---|
| Language: | English |
|---|
| References: | 35 |
|---|
| Note: | Figures
Tables |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, L. H., P. Ozias-Akins, G. Kochert, S. Kresovich, R. Dean, and W. Hanna. 1999. Differentiation of bermudagrass (Cynodon spp.) genotypes by AFLP analyses. Theor. Appl. Genet. 98:p. 895-902. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=66690 |
| If there are problems with this record, send us feedback about record 66690. |
| Choices for finding the above item: |
|---|
| Web URL(s):
https://link.springer.com/article/10.1007%2Fs001220051148
Last checked: 09/27/2017 |
| 
MSU catalog number: QH 426 .T47 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
