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| Publication Type:
| Report |
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| Author(s): | Yuen, G. Y.;
Zhang, Z. |
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| Author Affiliation: | Department of Plant Pathology, University of Nebraska |
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| Title: | Brown patch control with Stenotrophomonas maltophilia C3, 1999 |
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| Section: | Turfgrass
Other records with the "Turfgrass" Section
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| Source: | Biological and Cultural Tests for Control of Plant Diseases. 2000, p. 54. |
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| Publishing Information: | St. Paul, MN: The American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Brown patch; Rhizoctonia solani; Festuca arundinacea; Lolium perenne; Disease control; Biological control; Stenotrophomonas maltophilia; Disease severity; Formulations; Comparisons; Phosphates
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| Cultivar Names: | Manhattan; Kentucky 31 |
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| Abstract/Contents: | "Stenotrophomonas maltophilia strain C3 is a chitinolytic bacterium isolated from Kentucky bluegrass foliage. It was shown to have little efficacy against brown patch in previous field experiments when the bacterium was applied as cells grown on agar media suspended in buffer. In this study, we evaluated the benefits of applying whole cultures of strain C3 grown in a broth medium having chitin as the carbon source. Experiments were conducted on established swards at the University of Nebraska John Seaton Anderson Turfgrass Research Facility near Mead, NE. The plots were maintained under low fertility (around 50 kg N/ha annually), irrigated twice per wk, and mowed at a height of 8 cm. Urea (50 kg/ha) was applied to the experiment areas about 2 wk before the experiments. Brown patch activity was preexistent in both areas, but to ensure inoculum uniformity, 40 g of seed inoculum, containing two Nebraska isolates of R. solani AG1-IA, were in a block design. Treatments were: 1) C3 cells from tryptic soy agar suspended in phosphate buffer; 2) whole 7-day-old cultures of C3 in chitin broth, diluted 1:1 with water; 3) diluted (1:1, v/v), filter-sterilized fluid from C3 chitin broth cultures; and 4) nontreated control. C3 treatments contained approximately 5x10⁸ CFU/ml. The three liquid treatments were amended with 0.25% (v/v) Soydex as a spreader-sticker. Each treatment was applied twice (14 Aug and 20 Aug) at 500 ml per plot each time. On 1 Sept, all plots were rated for brown patch severity (1 to 5 scale: 1 = no disease, 5 = > 80% of turf area blighted) and for quality (1 to 10 scale: 1 = lowest, 10 = highest). Disease levels in nontreated plots in both grass species were moderate to high. All of the treatments reduced brown patch severity and improved turf quality in comparison to the nontreated control. Cell-free culture fluid was as effective as C3 suspended in phosphate buffer. The C3 chitin broth culture treatment was most effective in suppressing brown patch in both experiments." |
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| Language: | English |
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| References: | 0 |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yuen, G. Y., and Z. Zhang. 2000. Brown patch control with Stenotrophomonas maltophilia C3, 1999. Biol. Cult. Tests Control Plant Dis. p. 54. |
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MSU catalog number: SB 975 .B5 |
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