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| Web URL(s): | http://www.sciencedirect.com/science/article/pii/S1049964499907028/pdf?md5=c7152445a2a1d2f129a90b4e72487e88&pid=1-s2.0-S1049964499907028-main.pdf
Last checked: 09/29/2015
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| Publication Type:
| Refereed |
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| Author(s): | Wilson, Michael;
Xin, Wanmin;
Hashmi, Sarwar;
Gaugler, Randy |
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| Author Affiliation: | Department of Entomology, Rutgers University, New Brunswick, New Jersey |
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| Title: | Risk assessment and fitness of a transgenic entomopathogenic nematode |
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| Source: | Biological Control: Theory and Application in Pest Management. Vol. 15, No. 1, May 1999, p. 81-87. |
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| Publishing Information: | San Diego: Academic Press |
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| # of Pages: | 7 |
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| Related Web URL: | http://www.sciencedirect.com/science/article/pii/S1049964499907028
Last checked: 09/29/2015
Notes: Abstract only |
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| Keywords: | TIC Keywords: Entomopathogenic nematodes; Heterorhabditis bacteriophora; Risk assessment; Transgenes; Nematoda; Parasites; Genetic engineering; Heat resistance; Infectivity; Temperatures
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| Abstract/Contents: | "A strain of the entomopathogenic nematode, Heterorhabditis bacteriophora, was transformed by the addition of a heat-shock protein gene (hsp70A) from the free-living nematode Caenorhabditis elegans. Laboratory experiments with wild-type and transgenic nematodes were done at 16, 25, 30, and 37°C to investigate infectivity, reproductive capacity, and survival in water. Heat-shocked transgenic nematodes (ones subjected to a heat shock that is lethal to wild-type nematodes) were included in experiments to determine if this treatment caused sublethal damage. We found no significant differences in the ability of wild-type, transgenic, and heat-shocked-transgenic nematodes to infect or to reproduce in last-instar Galleria mellonella larvae or to srvive at these temperatures. We compared the ability of wild-type and transgenic nematodes to infect and kill 11 species of invertebrates, representing groups known to be susceptible and nonsusceptible to wild-type nematodes. Transformation caused no detectable change in virulence of nematodes to any test invertebrates. Transgenic and wild-type nematodes were also injected interperitoneally into mice and transgenic nematodes were fed by injection to mice without mouse mortality or changes in growth rate. We conclude that genetic engineering has provided a precise method to alter the heat shock tolerance of H. bacteriophora without affecting other important life history characteristics and that the transgenic nematode is unlikely to pose any threat to the environment if released on a wide scale." |
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| Language: | English |
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| References: | 35 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wilson, M., W. Xin, S. Hashmi, and R. Gaugler. 1999. Risk assessment and fitness of a transgenic entomopathogenic nematode. Biol. Control: Theory Appl. Pest Manage. 15(1):p. 81-87. |
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| Web URL(s):
http://www.sciencedirect.com/science/article/pii/S1049964499907028/pdf?md5=c7152445a2a1d2f129a90b4e72487e88&pid=1-s2.0-S1049964499907028-main.pdf
Last checked: 09/29/2015
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Access conditions: Item is within a limited-access website |
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MSU catalog number: SB 925 .B5 |
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