| |
| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2000.84.9.947
Last checked: 08/27/2010
Requires: PDF Reader |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Fouly, H. M.;
Wilkinson, H. T. |
|---|
| Author Affiliation: | Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, Urbana |
|---|
| Title: | Detection of Gaeumannomyces graminis varieties using polymerase chain reaction with variety-specific primers |
|---|
| Source: | Plant Disease. Vol. 84, No. 9, September 2000, p. 947-951. |
|---|
| Publishing Information: | St. Paul, MN: American Phytopathological Society |
|---|
| # of Pages: | 5 |
|---|
| Keywords: | TIC Keywords: Polymerase chain reaction; Gaeumannomyces graminis var. graminis; Gaeumannomyces graminis var. avenae; Gaeumannomyces graminis var. tricici; Take-all patch; DNA; Pathogens; DNA amplification; Disease identification; RNA polymerase; Simple sequence repeats; Genetic markers; Techniques
|
|---|
| Abstract/Contents: | "The polymerase chain reaction (PCR) was used for detection of Gaeumannomyces graminis, the causal agent of take-all disease in wheat, oats, and turfgrass. NS5 and NS6 universal primers amplified the middle region of 18S ribosomal DNA of Gaeumannomyces species and varieties. Primers GGT-RP (5' TGCAATGGCTTCGTGAA 3') and GGA-RP (5' TTTGTGTGTGACCATAC 3') were developed by sequence analysis of cloned NS5-NS6 fragments. The primer pair NS5-GGT-RP amplified a single 410-bp fragment from isolates of G. graminis var. tritici, a single 300-bp fragment from isolates of G. graminis var. avanae, and no amplification products from isolates of G. graminis var. graminis or other species of Gaeumannomyces. The primer pair NS5-GGA-RP amplified a single 400bp fragment from isolates of varieties tritici and avenae. Two sets of primer pairs (NS5:GGT-RP and NS5:GGA-RP) were used in PCR reactions to detect and identify the varieties tritici and avenae either colonizing wheat, oats, or grass roots, or in culture. No amplification products were observed using DNA extracted from plants infected with eight other soilborne fungal pathogens or from uninoculated plants. |
|---|
| Language: | English |
|---|
| References: | 32 |
|---|
| Note: | Figures
Tables |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Fouly, H. M., and H. T. Wilkinson. 2000. Detection of Gaeumannomyces graminis varieties using polymerase chain reaction with variety-specific primers. Plant Dis. 84(9):p. 947-951. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=67570 |
| If there are problems with this record, send us feedback about record 67570. |
| Choices for finding the above item: |
|---|
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2000.84.9.947
Last checked: 08/27/2010
Requires: PDF Reader |
| 
MSU catalog number: SB 599 .P95 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
