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| Web URL(s): | http://www.turf.rutgers.edu/research/abstracts/symposium1995.pdf#page=25
Last checked: 11/28/2007
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Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Bunting, Tracy E.;
Clarke, Bruce B.;
Hillman, Bradley I. |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University, New Brunswick, NJ |
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| Title: | Phylogenetic relationship of Magnaporthe spp. and other pathogenic fungi of the turf environment |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 5-6, 1995 |
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| Source: | Proceedings of the Fourth Annual Rutgers Turfgrass Symposium. 1995, p. 30. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Phylogeny; Magnaporthe; Genetic transformation; Fungal diseases; Summer patch; Disease identification; DNA amplification; Genetic markers; Poa pratensis; Disease resistance
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| Abstract/Contents: | "Magnaporthe poae is the causal agent of summer patch disease of bluegrasses and fine fescues. This patch disease is difficult to diagnose due to the lack of distinctive fungal structures in nature. Previously the only definite diagnosis involved mating the fungus in culture, which could take months to achieve. We have produced a method of identifying this fungus utilizing the polymerase chain reaction (PCR) technique. Using this method we are able to amplify DNA specifically from M. poae and M. rhizophila. The specific DNA is not amplified from the other thirty species of fungi tested. Because M. rhizophila is not found in North America, this method can be used to identify M. poae for definitive diagnosis of summer patch. Because M. poae and M. rhizophila share morphological traits, such as similar dark hyphae which grow on C3 grass hosts and similar ascospores and perithecia, we were interested in the relationship of M. poae to other species of Magnaporthe. In addition, the cloned DNA fragment of M. poae used to produce the primers hybridized to an isolate of Colletotrichum (Glomerella) graminicola in a Southern hybridization. The isolates we focused on for this study were: M. poae (3 isolates), M. rhizophila, M. salvinii, M. grisea (2 isolates), Gaeumannomyces graminis var. graminis, Gaeumannomyces incrustans, C. graminicola, and Leptosphaeria (Ophiosphaerella) korrae. The spacer region of the nuclear ribosomal DNA was used for the phylogenetic analysis of these fungi because universal amplification primers have been designed which can be used to amplify DNA from a wide range of organisms, and this locus has been used previously for phylogenetic analyses. The sequence analysis, using maximum parsimony, branch-and-bound algorithms of PAUP software, placed M. poae and M. rhizophila isolates in a monophyletic group. None of the turf pathogens formed strong unexpected monophylogenies (i.e. across genera.) The analysis supports the taxonomic placement of the G. incrustans, G. g. var. graminis, and M. poae as distinct species. The close phylogenetic relationship between M. poae and M. rhizophila explains the difficulty in producing a DNA detection method that would identify all isolates of M. poae without detecting M. rhizophila. Currently we are developing a method to transform M. poae with foreign DNA. This method involves producing fungal protoplasts through digestion of the cell wall, introduction of DNA, and selection of transformants using benomyl resistance. This transformation method will aid in studying the effects of potential avirulence genes and in "marking" the fungus for better viewing of the infection process. In addition, we are currently screening cultivars of Kentucky bluegrass for variations of resistance to isolates of M. poae which may help in studying virulence factors of the fungus." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bunting, T. E., B. B. Clarke, and B. I. Hillman. 1995. Phylogenetic relationship of Magnaporthe spp. and other pathogenic fungi of the turf environment. Proc. Annu. Rutgers Turfgrass Symp. p. 30. |
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| Web URL(s):
http://www.turf.rutgers.edu/research/abstracts/symposium1995.pdf#page=25
Last checked: 11/28/2007
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 433 .R88 |
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