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| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1985Articles/phyto75n11_1275.pdf#page=56
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Welty, R. E.;
Azevedo, M. D.;
Cook, K. L. |
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| Author Affiliation: | USDA ARS, Dept. Botany and Plant Pathology, Oregon State University |
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| Title: | Detecting hyphae of endophytic fungi in seedling meristems of perennial ryegrass and tall fescue |
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| Source: | Phytopathology. Vol. 75, No. 11, November 1985, p. 1331. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| Keywords: | TIC Keywords: Lolium perenne; Festuca arundinacea; Acremonium loliae; Acremonium coenophialum; Captafol; Endophytes; Alternaria; Cladosporium; Epicoccum
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| Abstract/Contents: | "A test was developed to detect endophyte (Acremonium loliae and A. coenophialum) hyphae in meristems and leaf sheaths of perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea). Seeds were soaked 1 hr in captafol (4F) at 14,780 ug/ml or 7,390 ug/ml in deionized water, air-dried overnight, and germinated (7 days at 5 C in dark, followed by 14 days at 8 hr light 25 C and 16 hr dark at 15 C). Seedlings were digested 16-18 hr in 5% NaOH with 0.1% trypan blue, boiled 20 min in lactophenol with 0.1% trypan blue, squash-mounted, and examined at x200. Captafol at 7,390 ug/ml reduced seedborne species of Alternaria, Cladosporium, and Epicoccum, allowed growth of A. coenophialum and A. loliae in meristems or leaf sheaths, and caused no phytotoxicity. Captafol at 7,390 ug/ml stunted roots. The technique provides a rapid method for detecting viable endophytes in these grass species." |
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| Language: | English |
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| References: | 0 |
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| Note: | "Abstracts of presentations at the 1985 Annual Meeting of the American Phytopathological Society."
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Welty, R. E., M. D. Azevedo, and K. L. Cook. 1985. Detecting hyphae of endophytic fungi in seedling meristems of perennial ryegrass and tall fescue. Phytopathology. 75(11):p. 1331. |
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| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1985Articles/phyto75n11_1275.pdf#page=56
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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