Full TGIF Record # 70957
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Yu, T. T.; Huang, B. R.; Skinner, D. Z.; Muthukrishnan, S.; Liang, G. H.
Author Affiliation:Kansas State University
Title:Genetic transformation of creeping bentgrass
Section:Turfgrass science
Other records with the "Turfgrass science" Section
Meeting Info.:Minneapolis, MN: November 5-9, 2000
Source:2000 Annual Meeting Abstracts [ASA/CSSA/SSSA]. 2000, p. 154.
# of Pages:1
Publishing Information:[Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America
Keywords:TIC Keywords: Genetic transformation; Agrostis stolonifera; Herbicide resistance; Fungi; Pathogens; Resistance; Agrobacterium tumefaciens; Genes; Calli; Bialaphos
Abstract/Contents:"Using biolostic bombardment, it has been known that genes resistant to herbicide and fungal pathogens can be incorporated into creeping bentgrass. It has also been demonstrated that Agrobacterium transormation is feasible for creeping bentgrass where GFP was used as a reporter gene. The efficiency (GFP+calli/total co-cultured calli) of Agrobacterium-mediated transformation from two independent trials was 7% and 10%, respectively. In this experiment, we co-bombarded the calli derived from bentgrass (cv Crenshaw) with plasmid DNA containing chi11, a gene resistant to fungal diseases linked to the bar gene, and plasmid DNA containing GFP, the gene encoding the green-fluorescent protein, in an attempt to introduce resistance to creeping bentgrass and to compare the efficiency with that of Agrobacterium-mediated transformation. A total of 214 pieces of calli were co-bombarded, one showed green fluorescence that also regenerated from selection using 4 mg/L of bialaphos (gfp+/bial+), one regenerated from bialaphos selection with no GFP expression (gfp-/bial+), and two showed strong GFP but could not produce seedlings under bialaphos selection (gfp+/bial-). All putative transgenic seedlings were sprayed with 0.2% Liberty solution and nine of the survival ones were randomly chosen for analysis by Southern blot. Results indicated that all nine seedlings showed clear bands corresponding to the chitinase coding region (1.1 Kb)."
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yu, T. T., B. R. Huang, D. Z. Skinner, S. Muthukrishnan, and G. H. Liang. 2000. Genetic transformation of creeping bentgrass. Annu. Meet. Abstr. p. 154.
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