Full TGIF Record # 71273
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Publication Type:
Content Type:Abstract or Summary only
Author(s):Meyer, W.; Zhang, G. Y.; Lu, S.; Chen, S. Y.; Chen, T. A.; Funk, R.
Author Affiliation:Meyer, Zhang, Lu, Funk: Rutgers University; Chen: Institute of Genetics, Chinese Academy of Science
Title:Transformation of Kentucky bluegrass (Poa pratensis L.) with betaine aldehyde dehydrogenase gene for salt and drought tolerance
Section:Turfgrass science
Other records with the "Turfgrass science" Section
Meeting Info.:Minneapolis, MN: November 5-9, 2000
Source:2000 Annual Meeting Abstracts [ASA/CSSA/SSSA]. 2000, p. 167.
# of Pages:1
Publishing Information:[Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America
Keywords:TIC Keywords: Apomixis; Poa pratensis; Breeding improvement; Genetic engineering; Genes; Salt tolerance; Drought resistance
Abstract/Contents:"The characteristic of apomixis makes Kentucky bluegrass a good target for genetic improvement by biotechnology. Particle bombardment was used to transform BADH gene into seed-derived callus from the Kentucky bluegrass variety, Langara. Cotransformation method was used to transform Hyg resistance gene and BADH gene into the callus, which were driven by rice actin promoter and maize ubiquitin promoter, respectively. After screening and regeneration on 200mg/L Hyg added medium, 65 Hyg resistant transgenic plants, which came from 81 different Hyg resistant callus lines, were obtained. PCR assay identified 43 transgenic lines contained BADH gene. Northern blots indicated the full-length transcription of BADH gene. BADH activity in some transgenic plants was 1.5-7.6 times higher than control. Tests in green house showed the salt and drought tolerance of 3 transgenic lines was significantly higher than control. In the process of tansformation [transformation] and regeneration, abundant somaclonal variation was observed."
Note:This item is an abstract only!
See Also:Other items relating to: SALTT
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Meyer, W., G. Y. Zhang, S. Lu, S. Y. Chen, T. A. Chen, and R. Funk. 2000. Transformation of Kentucky bluegrass (Poa pratensis L.) with betaine aldehyde dehydrogenase gene for salt and drought tolerance. Annu. Meet. Abstr. p. 167.
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