Full TGIF Record # 71929
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Web URL(s):http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2494.2000.00235.x/epdf
    Last checked: 10/02/2015
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Publication Type:
i
Refereed
Author(s):Bai, Y.; Qu, R.
Author Affiliation:Department of Crop Science, North Carolina State University, Raleigh, NC
Title:An evaluation of callus induction and plant regeneration in twenty-five turf-type tall fescue (Festuca arundinacea Schreb.) cultivars
Source:Grass and Forage Science. Vol. 55, No. 4, December 2000, p. 326-330.
# of Pages:5
Publishing Information:Oxford, Blackwell Scientific Publications
Keywords:TIC Keywords: Cultivar evaluation; Calli; Regeneration; Festuca arundinacea; Genetic variability; Genetic transformation; Tissue culture; Choice of cultivar; Genetic engineering; Seeds; Synthetic seeds; Cultivar variation; Transgenic turfgrasses
Abstract/Contents:"In order to investigate the genetic variation in tissue culture response and to find the cultivars with high regeneration ability for genetic transformation, twenty-five turf-type tall fescue (Festuca arundinacea Schreb.) cultivars, including many of the elite ones released recently, were evaluated for their callus induction and plant regeneration responses. Callus induction was initiated from mature seeds on a Murashige and Skoog (MS) medium containing 9.0 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D). Induced calli were subcultured on the same medium with 2.0 mg l-1 2,4-D and then transferred to a MS medium supplemented with 2.5 mg l-1 6-benzylaminopurine (BAP) for plant regeneration. Significant differences were observed among the twenty-five cultivars in both callus induction and plant regeneration (P < 0.001). Callus induction rate of viable seeds varied from 4.4% to 51.9%. Callus regeneration rates ranged from 16.7% to 58.8%. Overall regeneration rates (number of regenerated calli over number of cultured viable seeds) ranged from 1% to 22%. Approximately 94% of the regenerents were green plantlets."
Language:English
References:17
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bai, Y., and R. Qu. 2000. An evaluation of callus induction and plant regeneration in twenty-five turf-type tall fescue (Festuca arundinacea Schreb.) cultivars. Grass Forage Sci. 55(4):p. 326-330.
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Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2494.2000.00235.x/epdf
    Last checked: 10/02/2015
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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