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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2000.90.6.S1#page=54
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Mullins, E. D.;
Romaine, P.;
Chen, X.;
Geiser, D. M.;
Raina, R.;
Kang, S. |
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| Author Affiliation: | Mullins, Romaine, Chen, Geiser, and Kang: Department of Plant Pathology; and Raina: Department of Biology, The Pennsylvania State University, PA |
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| Title: | Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum: A valuable tool for tagging pathogenicity genes |
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| Section: | Abstracts submitted for presentation at the 2000 APS Annual meeting
Other records with the "Abstracts submitted for presentation at the 2000 APS Annual meeting" Section
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| Meeting Info.: | APS Annual Meeting, New Orleans, LA, August 12-16, 2000 |
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| Source: | Phytopathology. Vol. 90, No. 6, June 2000, p. S54. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrobacterium tumefaciens; Fusarium oxysporum; Genetic transformation
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| Abstract/Contents: | "Many fungi pathogenic on Arabidopsis have been identified and a wealth of information exists on the defense responses deployed by the host. However, none of these pathogens have been characterized with respect to the molecular basis of their pathogenicity on Arabidopsis. We applied an insertional mutagenesis technique based on Agrobacterium tumefaciens-mediated transformation to identify Fusarium oxysporum genes, important for pathogenicity on Arabidopsis. Conidia of a F. oxysporum isolate, highly virulent on Arabidopsis, were transformed and a frequency approximating to 300 transformants per 106 conidia was attained. The majority of transformants possessed a single T-DNA copy. Transformants were assayed for change-of-virulence on the most susceptible ecotype, Cape Verde. A TAIL-PCR strategy was employed to isolate those DNA fragments flanking T-DNA. The transformation protocol and results of the mutant screening will be presented." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Mullins, E. D., P. Romaine, X. Chen, D. M. Geiser, R. Raina, and S. Kang. 2000. Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum: A valuable tool for tagging pathogenicity genes. Phytopathology. 90(6):p. S54. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2000.90.6.S1#page=54
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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