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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2000.90.6.S1#page=70
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Schoedel, B. A.;
Maroon, C. J. M. |
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| Author Affiliation: | Agdia, Inc. Elkhart, IN |
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| Title: | Evaluation of extraction methods for improved detection of Xanthomonas campestris pv. pelargonii by PCR |
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| Section: | Abstracts submitted for presentation at the 2000 APS annual meeting
Other records with the "Abstracts submitted for presentation at the 2000 APS annual meeting" Section
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| Meeting Info.: | APS Annual Meeting, New Orleans, LA, August 12-16, 2000 |
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| Source: | Phytopathology. Vol. 90, No. 6, June 2000, p. S70. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Xanthomonas campestris; Polymerase chain reaction; Methodology; DNA; Comparisons; Disease identification; Techniques
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| Abstract/Contents: | "In previous trials, routine PCR detection of Xanthomonas campestris pv. pelargonii (Xcp) in geraniums resulted in inconsistent sensitivity. The problem with sensitivity was partly due to the presence of putative plant-derived substances that inhibited PCR. In an effort to reduce this inhibition, various methods of extracting bacteria from plants were combined with several DNA isolation methods. Liquid nitrogen, the Meku Juice-Press, soa, and smash methods of extraction were tried. These were combined with four different DNA isolation methods: NaOH method, DNA release method (Gene Releaser, Bio Ventures), boil method, affinity binding and selective elution method (DNeasy Plant Kit, Qiagen). Of these methods, the affinity binding and selective elution method was consistently highly effective. When this method of DNA isolation was used on dilutions of sap from Xcp-infected geraniums, PCR using the primer pair, XcpM1/XcpM2 (Sulzinkski et al., 1996), was 40-fold more sensitive than ELISA." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Schoedel, B. A., and C. J. M. Maroon. 2000. Evaluation of extraction methods for improved detection of Xanthomonas campestris pv. pelargonii by PCR. Phytopathology. 90(6):p. S70. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2000.90.6.S1#page=70
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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