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DOI: | 10.21273/HORTSCI.34.3.456E |
Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/34/3/article-p456E.xml?rskey=INJO7y Last checked: 11/15/2019 Requires: PDF Reader Notes: Item is within a single large file; Abstract only |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Ahn, B. J.;
Choi, J. S.;
Kamo, K.;
King, J. W. |
Author Affiliation: | Ahn, Choi: Dankook University, Cheonan, Korea;Kamo: USDA, ARS, FNPRU, Beltsville, MD;King: Department of Horticulture, University of Arkansas, Fayetteville, AR |
Title: | Biolistic transformation of zoysiagrass for gluphosinate resistance |
Section: | Genetics/Breeding/Biotechnology Other records with the "Genetics/Breeding/Biotechnology" Section
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Meeting Info.: | 96th Annual International Conference of the American Society for Horticultural Science, Minneapolis, MN: July 27-31, 1999 |
Source: | HortScience. Vol. 34, No. 3, June 1999, p. 456-457. |
Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
# of Pages: | 2 |
Keywords: | TIC Keywords: Biolistic transformation; Zoysia; Herbicide resistance; Caryopses; Radiation; Transgenic turfgrasses
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Abstract/Contents: | "Biolistic transformation methods for zoysiagrasses (Korean lawngrass) were developed and used to introduce a herbicide-resistant trait. Embryogenic calli were induced from mature caryopses on MS medium supplemented with 2 mg/L of 2,4-D, and used to establish liquid agitation cultures. The cultures have been maintained over a year without loss of the embryogenic competence. A particle bombardment method was optimized for zoysiagrass based on transient gusA gene expression. The most transient GUS expression upon bombardment treatments occurred at 110 psi of helium pressure with 10 cm of particle flying distance and 0.125 M sorbitol preculture treatment. Promoters suitable for zoysiagrass were compared, and actin and ubiquitin promoters were found effective in expressing gusA gene. Vector DNAs containing a herbicide resistant gene (bar), pBY505, were introduced into embryogenic cells of zoysiagrass using the optimized method. Total 194 putatively transformed plants were regenerated from 60 biolistic plates for over 6 months through selection culture containing 4-10 mg/L of phosphinothricin. Regenerants were grown in potting soil in greenhouse and sprayed with 1.7 g/L of Ignite herbicide. The transgenic plants showed various levels of resistance to the herbicide, while untransformed control plants were all dead. Recombination of the bar gene into the genomes of the transformants were confirmed through PCR and Southern blot analysis." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Ahn, B. J., J. S. Choi, K. Kamo, and J. W. King. 1999. Biolistic transformation of zoysiagrass for gluphosinate resistance. HortScience. 34(3):p. 456-457. |
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| DOI: 10.21273/HORTSCI.34.3.456E |
| Web URL(s): https://journals.ashs.org/hortsci/view/journals/hortsci/34/3/article-p456E.xml?rskey=INJO7y Last checked: 11/15/2019 Requires: PDF Reader Notes: Item is within a single large file; Abstract only |
| MSU catalog number: SB 1 .H64 |
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