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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2000.pdf#page=37
Last checked: 12/03/2007
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Kobayashi, Donald Y.;
Palumbo, Jeff;
Reedy, Ralph;
El-Barrad, Nour |
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| Author Affiliation: | Department of Plant Pathology, Rutgers University |
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| Title: | Extracellular enzyme production and biological control activity is regulated by a catabolite activator protein (CAP) homolog in the bacterium, C3, a taxonomically-related strain to Lytobacter mycophilus (proposed Gen. nov., sp. nov) strain N4-7 |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | Cook College, Rutgers University: January 13-14, 2000 |
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| Source: | Proceedings of the Ninth Annual Rutgers Turfgrass Symposium. 2000, p. 36. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Enzymes; Biological control; Lytobacter mycophilus; Taxonomy; Poa pratensis; Brown patch; Summer patch; Rhizoctonia solani; Magnaporthe poae; Bacteria; Chitinase; Esterase
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| Abstract/Contents: | "Bacterial strain C3 was previously isolated from a leaf of Kentucky bluegrass in Nebraska, and demonstrated biocontrol efficacy against brown patch disease caused by Rhizoctonia solani (Giesler and Yuen, 1998. Crop Prot. 17:509-513). Bacterial strain N4-7 was isolated in New Jersey and was demonstrated to have biocontrol activity against summer patch disease caused by Magnaporthe poae (Kobayashi and El-Barrad, 1996. Cur. Microbiol. 32:106-110). 16s rDNA sequence analysis, fatty acid methyl ester analysis and phenotypic comparisons indicated that strain C3 is taxonomically related to strain N4-7, but is significantly different from other described bacteria to warrant taxonomic positioning as a new genus and species. In an effort to understand the mechanisms involved in biocontrol activity, strain C3 was subjected to random transposon mutagenesis. A single mutant of strain C3, designated 5E4, was isolated that is devoid of chitinase, glucanase, and esterase activity, and is reduced in extracellular protease activity. Molecular characterization of mutant 5E4 indicated a transposon insertion in a gene that has significant nucleotide sequence identity to the catabolite activator protein (CAP) of Escherichia coli, Erwinia chrysanthemi and Xanthomonas campestris. Biological assays indicated mutant 5E4 is affected in in vitro antifungal activity against M. poae and R. solani, is no longer able to colonize M. poae mycelia, and is reduced in biocontrol activity of summer patch on Kentucky bluegrass. These studies provide the first step towards understanding the antifungal traits expressed by strain C3, and the regulatory pathway that controls biocontrol activity." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Kobayashi, D. Y., J. Palumbo, R. Reedy, and N. El-Barrad. 2000. Extracellular enzyme production and biological control activity is regulated by a catabolite activator protein (CAP) homolog in the bacterium, C3, a taxonomically-related strain to Lytobacter mycophilus (proposed Gen. nov., sp. nov) strain N4-7. Proc. Annu. Rutgers Turfgrass Symp. p. 36. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2000.pdf#page=37
Last checked: 12/03/2007
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 433 .R88 |
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