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Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2001.91.6.S129#page=6 Last checked: 10/16/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Floyd, R. M.;
Papert, A.;
Blaxter, M. L. |
Author Affiliation: | ICAPB, University of Edinburgh |
Title: | Molecular barcodes for soil nematode identification |
Meeting Info.: | 2001 APS/MSA/SON Joint Meeting, Salt Lake City, Utah: August 25-29, 2001 |
Source: | Phytopathology. Vol. 91, No. 6, June Supplement 2001, p. S134. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Molecular biology; Nematoda; Identification; DNA; Polymerase chain reaction; Ribonucleic acid; Taxonomy; Genetic markers; Genetic diversity
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Abstract/Contents: | "We have developed a molecular barcoding system for identification of soil nematodes by DNA sequencing. PCR is carried out on individual nematodes, and the 5' segment of the small subunit ribosomal RNA (SSU) gene is amplified and sequenced. Resulting sequences (typically 450-500 bases) are aligned and clustered using a neighbour-joining algorithm. Groups of similar or identical sequences are designated as molecular operational taxonomic units (MOTU). A Scottich upland Agrostis-Festuca grassland soil was sampled, using both random and culture-based methods. The MOTU discovered could readily be assigned to classical, morphologically defined taxonomic groups using a database of SSU sequences from named nematode species. The MOTU technique allows a rapid assessment of nematode taxonomic diversity in soils. Correlation with a database of sequences from known species offers a route to application of the technique in ecological surveys addressing biological as well as genetic diversity." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Floyd, R. M., A. Papert, and M. L. Blaxter. 2001. Molecular barcodes for soil nematode identification. Phytopathology. 91(6):p. S134. |
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| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2001.91.6.S129#page=6 Last checked: 10/16/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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