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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2001.91.6.S129#page=16
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Tytgat, T.;
Vercauteren, I.;
De Meutter, J.;
Gheysen, G.;
Coomans, A. |
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| Author Affiliation: | Tytgat, Coomans: Institute of Zoology; Vercauteren, Meutter: Department of Plant Genetics; Gheysen: Faculty of Agriculture and Applied Biological Sciences, Universiteit Gent, L. L. Ledeganckstraat |
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| Title: | Identification of Meloidogyne incognita genes by differential display on infected roots |
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| Meeting Info.: | 2001 APS/MSA/SON Joint Meeting, Salt Lake City, Utah: August 25-29, 2001 |
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| Source: | Phytopathology. Vol. 91, No. 6, June Supplement 2001, p. S144. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Genes; Roots; Infection; Identification; DNA; Nematoda; Meloidogyne incognita; Ribonucleic acid
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| Abstract/Contents: | "Arabidopsis thaliana plants were inoculated with freshly hatched Meloidogyne incognita J2 juveniles. Root galls were harvested 2, 3, 4, 5 and 7 days post inoculation. Total RNA was isolated from the root galls and noninfected root tissue. After first strand synthesis, differential display analysis was performed with random decamers. 29 bands specific for gall tissue were isolated, reamplified and cloned. DNA sequence analysis revealed 75 different sequences. By southern blotting on genomic DNA, 6 clones were identified to be from nematode origin. None of them showed homology to any known gene sequence in the available databases. Currently, full length clones are isolated from a cDNA library, and in situ hybridisation is performed." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tytgat, T., I. Vercauteren, J. De Meutter, G. Gheysen, and A. Coomans. 2001. Identification of Meloidogyne incognita genes by differential display on infected roots. Phytopathology. 91(6):p. S144. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2001.91.6.S129#page=16
Last checked: 10/16/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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