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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/2001jou165.pdf
Last checked: 08/05/2010
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Fei, S.;
Yu, T.;
Clemente, T.;
Riordan, T. |
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| Author Affiliation: | Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE |
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| Title: | Nodal segment explant as a potential target for the genetic engineering of buffalograss |
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| Section: | Genetics and breeding
Other records with the "Genetics and breeding" Section
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| Meeting Info.: | Toronto, Ontario, Canada: July 2001 |
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| Source: | International Turfgrass Society Research Journal. Vol. 9, No. Part 1, 2001, p. 165-168. |
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| Publishing Information: | Oakville, Ontario, Canada: International Turfgrass Society |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: NAA; Application rates; Growth regulators; Node survival; Genetic engineering; Bouteloua dactyloides; Herbicide resistance; Regeneration; Tissue culture; Benzyladenine; Cutting methods; Particle bombardment
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| Cultivar Names: | 609 |
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| Abstract/Contents: | "The excellent drought tolerance of buffalograss [Buchloe dactyloides (Nutt.) Englem.] makes it a good choice for use as a low maintenance turfgrass. However, due to its slow growth rate, buffalograss is susceptible to weed infestations. Hence, herbicide resistance would be a valuable agronomic trait for bufflograss. A prerequisite of any gene transfer system in plants is an efficient regeneration protocol. Nodal segments of buffalograss cv '609' with pre-existing buds completely removed were cultured on MS medium containing 0, 0.1 or 0.5 mg 1-1 α-naphthaleneacetic acid (NAA) combined with 0.1, 0.5, 1 or 2 mg 1-1 6-benzladenine (BA). De novo shoot formation was optimal under a growth regulator regime of 0.1 mg 1-1NAA plus either 0.1 mg 1-1 BA or 0.5 mg 1-1 BA. Preculture of nodal segments with pre-existing buds attached took place on MS medium containing 0.5, 1.0, 3.0 or 5.0 mg 1-1 BA for a week before they were cut at either a high (~10mm) or low position (~5mm) relative to the node to a strong influence on bud proliferation rate with cutting at a high position yielding more axillary buds than did those segments from the low position. The top portion of the nodal section with multiple buds was removed to expose the apical meristems, which were then biolistically bombarded with gold particles coated with a construct containing the ß-glucuronidase (GUS) gene. Adjustments of shooting parameters aiming to maximize particle penetration, including the increase of rupture disk pressure from 1350 psi to 1800 psi and reduction of particle travel distance from 9 cm to 6 cm did not improve particle penetration." |
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| Language: | English |
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| References: | 4 |
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| Note: | Pictures, b/w
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Fei, S., T. Yu, T. Clemente, and T. Riordan. 2001. Nodal segment explant as a potential target for the genetic engineering of buffalograss. Int. Turfgrass Soc. Res. J. 9(Part 1):p. 165-168. |
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| Web URL(s):
https://archive.lib.msu.edu/tic/its/articles/2001jou165.pdf
Last checked: 08/05/2010
Requires: PDF Reader |
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MSU catalog number: SB 433 .I52 v. 9 |
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