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| Web URL(s): | https://archive.lib.msu.edu/tic/its/articles/2001jou198.pdf
Last checked: 08/05/2010
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| Publication Type:
| Refereed |
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| Author(s): | Qu, R.;
Chaudhury, A. |
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| Author Affiliation: | Qu: Department of Crop Science, North Carolina State University, Raleigh, NC; Chaudhury: Bioresources and Biotechnology Division, Tata Energy Research Institute, New Delhi, India |
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| Title: | Improved young inflorescence culture and regeneration of 'Tifway' bermudagrass (cynodon transvaalensis x C. dactylon) |
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| Section: | Genetics and breeding
Other records with the "Genetics and breeding" Section
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| Meeting Info.: | Toronto, Ontario, Canada: July 2001 |
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| Source: | International Turfgrass Society Research Journal. Vol. 9, No. Part 1, 2001, p. 198-201. |
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| Publishing Information: | Oakville, Ontario, Canada: International Turfgrass Society |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: Cynodon dactylon; Cynodon transvaalensis; Regeneration; Inflorescences; Tissue culture; Genetic transformation; Cultivar improvement; Embryogenesis; Polyvinl acetate; Ascorbic acid
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| Cultivar Names: | Tifway |
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| Abstract/Contents: | "'Tifway', an interspecific triploid hybrid [Cynodon transvaalensis Burtt-Davy x C. dactylon (L.) Pers], and its derivatives are important bermudagrass cultivars for golf courses and athletic fields in warmer temperate to tropical regions of the world. Development of a reliable 'Tifway' tissue culture protocol is necessary to facilitate genetic transformation of these bermudagrass cultivars for further improvement. The objective of this study was to find a way to induce embryogenic callus and to regenerate 'Tifway' plants. Callus induction from young inflorescence was hindered by necrosis of the explant in culture medium. This problem was overcome by dipping the explants in a liquid MS medium conteining 0.2% L-ascorbic acid prior to plating and by inclusion of 6 g L-1 polyvinylpolypyrrolidone (PVPP), a polyphenol adsorbent, in the culture medium. The callus induction rate was 53% when segments of immature inflorescences (less than 15 mm in length) were cultured under these conditions. Two green 'Tifway' plantlets were regenerated." |
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| Language: | English |
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| References: | 19 |
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| Note: | Pictures, b/w
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Qu, R., and A. Chaudhury. 2001. Improved young inflorescence culture and regeneration of 'Tifway' bermudagrass (cynodon transvaalensis x C. dactylon). Int. Turfgrass Soc. Res. J. 9(Part 1):p. 198-201. |
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https://archive.lib.msu.edu/tic/its/articles/2001jou198.pdf
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MSU catalog number: SB 433 .I52 v. 9 |
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