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| Web URL(s): | https://web.archive.org/web/20080907212847/http://www.virtual.clemson.edu/groups/turfornamental/tmi/Weedman/Dinitroaniline.pdf
Last checked: 04/06/2016
Requires: PDF Reader
Notes: Clemson University Turfgrass Program reprint |
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| Publication Type:
| Refereed |
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| Author(s): | Lowe, D. B.;
Swire-Clark, G. A.;
McCarty, L. B.;
Whitwell, T.;
Baird, W. V. |
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| Author Affiliation: | Horticulture Department, Poole Agriculture Center, Clemson University, Clemson, SC |
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| Title: | Biology and molecular analysis of dinitroaniline-resistant Poa annua L. |
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| Section: | Turfgrass weeds
Other records with the "Turfgrass weeds" Section
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| Meeting Info.: | Toronto, Ontario, Canada: July 2001 |
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| Source: | International Turfgrass Society Research Journal. Vol. 9, No. Part 2, 2001, p. 1019-1025. |
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| Publishing Information: | Oakville, Ontario, Canada: International Turfgrass Society |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Poa annua; Poa annua control; Herbicide resistance; Cultural methods; Herbicides; Dinitroaniline herbicides; Dinitroaniline resistance; Canopy; Shoot density; Prodiamine; Molecular biology; DNA; Ribonucleic acid; Root growth; Root weight; Mutations
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| Abstract/Contents: | "Annual bluegrass is a problem weed in intensively managed turf. Recent reports indicate that cultural practices resulted in the selection of resistance to the anti-microtubule dinitroaniline herbicides. Plants, either collected on site from Georgia and South Carolina or grown from seed purchased commercially or collected from North Carolina plants, were evaluated to identify resistant populations and compare relative levels of resistance; as well as vegetative and reproductive growth potential between populations. To accomplish this, plants were grown in greenhouses and a rooting bioassay using stem segments was developed. In addition, investigations into the molecular mechanism of resistance were initiated and focused on beta-tubulin. These experiments were accomplished using Southern blots, reverse transcriptase-polymerase chain reaction, and DNA sequence analysis. Over a 39-week period, vegetative growth of susceptible plants out-paced that of resistant plants. This was particularly true for canopy development (shoot numbers). Over the same time period, although resistant plants initially produced more inflorescences, there was not a consistent difference in reproductive growth between the biotypes. Also, in general, there was not a significant difference in seed germination between the biotypes. A gene family of five to eight members encodes beta-tubulin. No restriction fragment length polymorphisms were identified between susceptible and resistant plants. However, a number of missense point-mutations were identified. In one beta-tubulin sequence, isolated from a resistant biotype, a mutation of amino acid 241 (converting arginine to lysine) is homologous to a mutation that confers resistance to anti-microtubule fungicides, in a yeast beta-tubulin." |
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| Language: | English |
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| References: | 41 |
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| Note: | Pictures, b/w
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Lowe, D. B., G. A. Swire-Clark, L. B. McCarty, T. Whitwell, and W. V. Baird. 2001. Biology and molecular analysis of dinitroaniline-resistant Poa annua L.. Int. Turfgrass Soc. Res. J. 9(Part 2):p. 1019-1025. |
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| Web URL(s):
https://web.archive.org/web/20080907212847/http://www.virtual.clemson.edu/groups/turfornamental/tmi/Weedman/Dinitroaniline.pdf
Last checked: 04/06/2016
Requires: PDF Reader
Notes: Clemson University Turfgrass Program reprint |
| 
MSU catalog number: SB 433 .I52 v. 9 |
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