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Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Schibeci, A.;
Henry, R. J.;
Stone, B. A. |
Author Affiliation: | Department of Biochemistry, La Trobe University, Bundora, Vic, 3083, Australia |
Title: | Separation of subcellular organelles and the localization of B-glucan synthesis in protoplasts of ryegrass endosperm cells |
Source: | Plant Physiology. Vol. 27, No. 1, May 1983, p. 15. |
Publishing Information: | Lancaster, PA: American Society of Plant Physiologists |
Keywords: | TIC Keywords: Lolium multiflorum; Protoplasts; Ultracentrifugation; Endoplasmic reticulum
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Abstract/Contents: | "Subcellular organelles and membranes including the plasma membrane (P.M.), rough and smooth endoplasmic reticulum (RER and SER) and Golgimembranes (GM) have been isolated from protoplasts of ryegrass (Lolium multiflorum) endosperm cells grown in suspension culture. P.M., protein bodies and amyloplasts were first removed from the protoplast lysate (Schibeci et al., 1982, Biochem. J. 205, 511) and subsequently the remaining intracellular membranes were separated by ultracentrifugation on a linear sucrose density gradient in the presence and absence of Mg2+. Fractions enriched in P.M., SER, RER and GM were obtained. The fractions were characterized by enzyme marker analysis, eletron microscopy and polyacrylamide gel electrophoresis. B-Glucan synthases, assayed at 10 uM and 1 mM DUP-glucose, were associated with all the endomembrane components but enrichment was greatest in the Golgi membranes. A significant proportion was also found in a fraction banding at the top of the sucrose gradient." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Schibeci, A., R. J. Henry, and B. A. Stone. 1983. Separation of subcellular organelles and the localization of B-glucan synthesis in protoplasts of ryegrass endosperm cells. Plant Physiol. 27(1):p. 15. |
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