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Publication Type:
| Professional |
Content Type: | Abstract or Summary only |
Author(s): | Bai, Yuyu;
Qu, Rongda |
Author Affiliation: | Bai:Doctoral Student, Crop Science Department, North Carolina State University; Qu: Assistant Professor, Crop Science Department, North Carolina State University |
Title: | Genetic transformation of elite turf-type cultivars of tall fescue |
Section: | Research: ITS summary Other records with the "Research: ITS summary" Section
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Source: | North Carolina Turfgrass. Vol. 18 [19], No. 6, October/November 2001, p. 41. |
Publishing Information: | Southern Pines, NC: The Turfgrass Council of North Carolina, Inc. |
# of Pages: | 1 |
Keywords: | TIC Keywords: Genetic transformation; Festuca arundinacea; Transferases; Calli; Resistance; Transgenic turfgrasses
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Cultivar Names: | Coronado; Virtue |
Abstract/Contents: | "Biotechnology approaches can supplement conventional breeding efforts for turfgrass improvement. The objective of this study was to develop reliable transformation and selectall fescue cultivars. Transgenic tall fescue (Festuca arundinacea Schreb.) plants were obtained by microprojectile bombardment of embryogenic suspension cells of two elite turf-type cultivars, Coronado and Virtue. Two selection systems were successfully used in the transformation. A bialaphos-based selection used plasmid pAHC25 that contains phosphinothricin acetyltransferase (bar) and b-glucuronidase (GUS) genes both driven by the maize ubi1 promoter. A two-step solid medium selection with increasing bialaphos concentration was used to obtain resistant callus. Alternatively, a hygromycin B (hyg B)-based selection strategy was employed using plasmid pAcH1 containing hygromycin phosphotransferase (hph) gene driven by the rice Act 1 gene promoter. A three-step liquid medium or a two-step solid medium selection was used to obtain resistant callus. Additional selection was used in the rooting medium eliminated escapes. Expression of the bar and GUS genes was demonstrated by resistance to the herbicide Finale and by GUS activity assays, respectively. The transgenic nature of the resistant plants was confirmed by Southern hybridization analysis." |
Language: | English |
References: | 0 |
Note: | Pictures, color This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Bai, Y., and R. Qu. 2001. Genetic transformation of elite turf-type cultivars of tall fescue. N.C. Turfgrass. 18 [19](6):p. 41. |
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