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| Publication Type:
| Professional |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Bradley, David E.;
Bruneau, Art H.;
Qu, Rongda |
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| Author Affiliation: | Bradley: Graduate Student, Crop Science Department, North Carolina State University; Bruneau: Professor and Extension Specialist, Crop Science Department, North Carolina State University; and Qu: Assitant Professor, Crop Science Department, North Carolina State University |
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| Title: | Effects of cultivar, explant treatment, and medium supplements on callus induction and plantlet regeneration in perennial ryegrass |
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| Section: | Research: ITS summary
Other records with the "Research: ITS summary" Section
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| Source: | North Carolina Turfgrass. Vol. 18 [19], No. 6, October/November 2001, p. 42. |
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| Publishing Information: | Southern Pines, NC: The Turfgrass Council of North Carolina, Inc. |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Lolium perenne; Genetic engineering; Tissue culture; Caryopses; Calli; Regeneration; Cytokinins; Copper; Cultivar evaluation; Cultivars
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| Cultivar Names: | Roadrunner |
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| Abstract/Contents: | "Perennial ryegrass (Lolium perenne L.) is a widely used cool-season grass species as turf and forage. In order to use biotechnological approaches to improve the species, factors affecting tissue culture responses of mature caryopses of perennial ryegrass were studied. Callus induction and regeneration from mature caryopses of 13 recently-released turf-type perennial ryegrass cultivars were evaluated, and significant differences were found among the cultivars with Roadrunner having the highest callus induction and regeneration rates. Callus induction rates increased more than five fold when the caryopses were longitudinally sliced before plating. Callus quality was also improved by this treatment resulting in substantially higher callus regeneration rates. Supplement of BAP (0.1- 0.5 mg L-1) in callus culture medium enhanced callus regeneration abilitu. Copper supplement up to 10 micromolars (mM) in the MS medium did not have any effect and 50 mM was toxic." |
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| Language: | English |
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| References: | 0 |
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| See Also: | Other items relating to: Biostimulants |
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| Note: | Pictures, color
This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bradley, D. E., A. H. Bruneau, and R. Qu. 2001. Effects of cultivar, explant treatment, and medium supplements on callus induction and plantlet regeneration in perennial ryegrass. N.C. Turfgrass. 18 [19](6):p. 42. |
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MSU catalog number: SB 433 .A1 N52 |
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