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Web URL(s): | http://archive.lib.msu.edu/tic/ressuml/266.pdf Last checked: 01/20/2017 Requires: PDF Reader |
Publication Type:
| Report |
Author(s): | Qu, Rongda |
Author Affiliation: | North Carolina State University |
Title: | Hybrid bermudagrass improvement by genetic transformation |
Section: | Turfgrass germplasm enhancement Other records with the "Turfgrass germplasm enhancement" Section
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Source: | 2001 Turfgrass and Environmental Research Summary. 2001, p. 27. |
Publishing Information: | Far Hills, NJ: United States Golf Association |
# of Pages: | 1 |
Keywords: | TIC Keywords: Hybrid bermudagrasses; Genetic transformation; Breeding improvement; Calli; Regeneration; Growth regulators; Biolistic transformation; Particle bombardment; Transgenic turfgrasses; Tissue culture; Cytokinins; Abscisic acid; Gibberellins; Somatic embryogenesis; Agrobacterium tumefaciens
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Language: | English |
References: | 0 |
See Also: | Other items relating to: Biostimulants |
See Also: | Other Reports from this USGA research project: 1998-18-126 |
Note: | Pictures, color |
USGA Summary Points: | Bermudagrass is recalcitrant in tissue culture and genetic transformation. Regeneration of bermudagrass plants through tissue culture has been further improved by adjusting plant hormones in the culture medium. A full course of secondary somatic embryogenesis has been photographed under a stereo microscope by tracking an embryogenic callus for over 70 days. Transformation procedures using 'gene gun' or Agrobacterium have been developed. A total of 19 putative transgenic callus lines were obtained by selection with hygromycin B (250mg/L) or bialaphos (5 mg/L). Some callus lines also showed GUS reporter gene activity. Transgenic nature of some lines have been confirmed by Southern Hybridization Analysis. |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Qu, R. 2001. Hybrid bermudagrass improvement by genetic transformation. Turfgrass Environ Res. Summ. p. 27. |
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| Web URL(s): http://archive.lib.msu.edu/tic/ressuml/266.pdf Last checked: 01/20/2017 Requires: PDF Reader |
| MSU catalog number: SB 433 .A1 A6 |
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