Full TGIF Record # 78361
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Web URL(s):http://archive.lib.msu.edu/tic/ressuml/266.pdf
    Last checked: 01/20/2017
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Publication Type:
i
Report
Author(s):Qu, Rongda
Author Affiliation:North Carolina State University
Title:Hybrid bermudagrass improvement by genetic transformation
Section:Turfgrass germplasm enhancement
Other records with the "Turfgrass germplasm enhancement" Section
Source:2001 Turfgrass and Environmental Research Summary. 2001, p. 27.
Publishing Information:Far Hills, NJ: United States Golf Association
# of Pages:1
Keywords:TIC Keywords: Hybrid bermudagrasses; Genetic transformation; Breeding improvement; Calli; Regeneration; Growth regulators; Biolistic transformation; Particle bombardment; Transgenic turfgrasses; Tissue culture; Cytokinins; Abscisic acid; Gibberellins; Somatic embryogenesis; Agrobacterium tumefaciens
Language:English
References:0
See Also:Other items relating to: Biostimulants
See Also:Other Reports from this USGA research project: 1998-18-126
Note:Pictures, color
USGA Summary Points:Bermudagrass is recalcitrant in tissue culture and genetic transformation. Regeneration of bermudagrass plants through tissue culture has been further improved by adjusting plant hormones in the culture medium. A full course of secondary somatic embryogenesis has been photographed under a stereo microscope by tracking an embryogenic callus for over 70 days. Transformation procedures using 'gene gun' or Agrobacterium have been developed. A total of 19 putative transgenic callus lines were obtained by selection with hygromycin B (250mg/L) or bialaphos (5 mg/L). Some callus lines also showed GUS reporter gene activity. Transgenic nature of some lines have been confirmed by Southern Hybridization Analysis.
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Qu, R. 2001. Hybrid bermudagrass improvement by genetic transformation. Turfgrass Environ Res. Summ. p. 27.
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http://archive.lib.msu.edu/tic/ressuml/266.pdf
    Last checked: 01/20/2017
    Requires: PDF Reader
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MSU catalog number: SB 433 .A1 A6
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