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| DOI: | 10.1006/bcon.2001.0986 |
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| Web URL(s): | http://dx.doi.org/10.1006/bcon.2001.0986
Last checked: 04/14/2008
Access conditions: Item is within a limted-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Wang, Xiaodong;
Grewal, Parwinder S. |
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| Author Affiliation: | Department of Entomology, The Ohio State University, OARDC, Wooster, Ohio |
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| Title: | Rapid genetic deterioration of environmental tolerance and reproductive potential of an entomopathogenic nematode during laboratory maintenance |
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| Source: | Biological Control: Theory and Application in Pest Management. Vol. 23, No. 2, January 2002, p. 71-78. |
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| Publishing Information: | San Diego: Academic Press |
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| # of Pages: | 8 |
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| Keywords: | TIC Keywords: Genetics; Entomopathogenic nematodes; Chilling; Desiccation; Ultraviolet radiation; Heat stress; Storage; Reproduction; Virulence; Heterorhabditis bacteriophora; Stress response
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| Abstract/Contents: | "Inadvertent selection is an important genetic problem frequently inflicted during laboratory culture and maintenance of biological control agents. Entomopathogenic nematodes can be maintained in the laboratory in three ways: cryopreservation in liquid nitrogen, storage at cold temperature with as few subcultures as possible, or storage at room temperature with frequent subcultures. We determined the influence of these laboratory maintenance regimes on the stress tolerance (UV, heat, and desiccation) and physiological fitness (storage stability, reproduction potential, and virulence) of a newly isolated strain GPS11 of Heterorhabditis bacteriophora. Except for desiccation tolerance, no detectable changes occurred with nematodes that were cryopreserved in liquid nitrogen for 1 year. Significant reduction in nematode heat, UV, and desiccation tolerance, storage stability, and reproductive potential occurred after only three passages in Galleria mellonella. The decline in nematode reproduction, storage ability, and stress tolerance was correlated with the increase in passage number. Increase in nematode virulence was observed when they were subcultured frequently in G. mellonella at room temperature. These results demonstrate that laboratory adaptation can produce dramatic changes in important biological attributes of entomopathogenic nematodes." |
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| Language: | English |
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| References: | 27 |
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| Note: | Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, X., and P. S. Grewal. 2002. Rapid genetic deterioration of environmental tolerance and reproductive potential of an entomopathogenic nematode during laboratory maintenance. Biol. Control: Theory Appl. Pest Manage. 23(2):p. 71-78. |
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| DOI: 10.1006/bcon.2001.0986 |
| Web URL(s):
http://dx.doi.org/10.1006/bcon.2001.0986
Last checked: 04/14/2008
Access conditions: Item is within a limted-access website |
| 
MSU catalog number: SB 925 .B5 |
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