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| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1993Articles/Phyto83n03_283.PDF
Last checked: 08/25/2010
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Henson, Joan M.;
Goins, Tresa;
Grey, William;
Mathre, Donald E.;
Elliott, Monica L. |
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| Author Affiliation: | Henson and Goins: Department of Microbiology; Grey and Mathre: Department of Plant Pathology, Montana State University, Bozeman; and Elliott: Fort Lauderdale Research and Education Center, University of Florida, Fort Lauderdale, FL |
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| Title: | Use of polymerase chain reaction to detect Gaeumannomyces graminis DNA in plants grown in artificially and naturally infested soil |
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| Section: | Molecular plant pathology
Other records with the "Molecular plant pathology" Section
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| Source: | Phytopathology. Vol. 83, No. 3, March 1993, p. 283-287. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: Polymerase chain reaction; Gaeumannomyces; DNA; Fungi; Take-all patch
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| Abstract/Contents: | "The polymerase chain reaction was used to specifically amplify DNA of Gaeumannomyces graminis, a filamentous soilborne fungus that causes root and crown disease of cereals and turfgrasses. Nested primers were used to amplify a 188-bp fragment of mitochondrial DNA from fungal and infected-plant samples, which were simply boiled to release target DNA. Fungal DNA was amplified from single boiled hyphal tips or ascospores. G. graminis DNA was also detected in boiled crowns and roots from experimentally and naturally infected wheat, rice, and St. Augustinegrass and experimentally infected bermudagrass. Inhibition of DNA amplification by soil was observed in reactions with relatively low copy numbers of target DNA sequences (i.e., up to 40 ascospores) but not in reactions with large numbers of target DNA sequences (i.e., entire perithecia). |
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| Language: | English |
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| References: | 40 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Henson, J. M., T. Goins, W. Grey, D. E. Mathre, and M. L. Elliot. 1993. Use of polymerase chain reaction to detect Gaeumannomyces graminis DNA in plants grown in artificially and naturally infested soil. Phytopathology. 83(3):p. 283-287. |
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| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1993Articles/Phyto83n03_283.PDF
Last checked: 08/25/2010
Requires: PDF Reader |
| 
MSU catalog number: SB 599 .P48 |
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