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| DOI: | 10.1093/aob/mcf024 |
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| Web URL(s): | https://academic.oup.com/aob/article/89/2/165/193261/
Last checked: 03/01/2017
Requires: PDF Reader
Notes: Guide page |
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| Publication Type:
| Refereed |
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| Author(s): | Espinoza, F.;
Pessino, S. C.;
Quarín, C. L.;
Valle, E. M. |
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| Author Affiliation: | Espinoza and Quarín: Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, UNNE, Corrientes, Argentina; Pessino and Valle: Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), División Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Rosario, Argentina |
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| Title: | Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD markers in Paspalum notatum |
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| Source: | Annals of Botany. Vol. 89, No. 2, February 2002, p. 165-170. |
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| Publishing Information: | London, Oxford University Press |
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| # of Pages: | 6 |
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| Keywords: | TIC Keywords: Growth analysis; Paspalum notatum; Physiological processes; Pollination; Reproduction; Timing
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| Abstract/Contents: | "Progeny tests employing molecular markers allow the identification of individuals originated by sexual means among the offspring of a facultative apomict. The objective of this work was to evaluate the effect of the pollination timing on the proportion of sexually formed individuals in progenies of a facultative apomictic Paspalum notatum genotype. Progeny families of approx. 30 plants each were generated at five different pollination times: 1-3 d pre-anthesis; at anthesis; and 2, 4 and 6 d post-anthesis. Cytoembryological analyses indicated that approx. 17% of the ovules carried a meiotic cytologically reduced embryo sac in florets formed simultaneously with those used for crosses. The parental plants and the five F1 families were analysed using RAPD molecular markers. Ninety-five oligonucleotides were assayed on the progenitors in order to search for male-specific bands. Eight primers presenting clear polymorphic bands were selected for use in the progeny tests. The proportion of sexually produced progeny reached 3·4% before anthesis and 20% at anthesis, while pollination after anthesis generated only maternal plants. A second progeny of 97 plants obtained from pollination at anthesis produced 16 off-type plants (16·5 %), of which only one was a BIII hybrid (2n + n). Our results indicate that pollination at anthesis allows the greatest potential for sexuality to be expressed in this facultative apomictic genotype. When pollution is delayed as soon as 2 d after anthesis, only the aposporous sacs develop endosperm through pseudogamy to set seed." |
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| Language: | English |
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| References: | 20 |
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| Note: | Pictures, b/w
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Espinoza, F., S. C. Pessino, C. L. Quarín, and E. M. Valle. 2002. Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD markers in Paspalum notatum. Ann. Bot. 89(2):p. 165-170. |
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| DOI: 10.1093/aob/mcf024 |
| Web URL(s):
https://academic.oup.com/aob/article/89/2/165/193261/
Last checked: 03/01/2017
Requires: PDF Reader
Notes: Guide page |
| 
MSU catalog number: QK 1 .A53 |
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