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| Web URL(s): | http://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1986Articles/PlantDisease70n05_380.pdf
Last checked: 10/13/2015
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Johnson, Mark C.;
Bush, Lowell P.;
Siegel, Malcom R. |
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| Author Affiliation: | University of Kentucky |
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| Title: | Infection of tall fescue with Acremonium coenophialum by means of callus culture |
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| Source: | Plant Disease. Vol. 70, No. 5, May 1986, p. 380-382. |
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| Publishing Information: | St. Paul, Minn., American Phytopathological Society |
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| Keywords: | TIC Keywords: Calli; Tissue culture; Festuca arundinacea; Acremonium coenophialum; Infection; Poa; Lolium perenne; Dactylis glomerata
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| Abstract/Contents: | "Calli initiated from peduncle tissue of endophyte-free tall fescue plants and maintained on a culture medium were inoculated with the endophytic fungus Acremonium coenophialum. This was accomplished by making an incision into the middle of calli 1-2cm in diameter and inserting a small amount (<1mm2) of mycelium followed by a 10-wk incubation. About 17% of the regenerated plants from these calli were infected as determined by microscopic examination of leaf sheaths and by enzyme-linked immunosorbent assay. Additional evidence for the successful establishment of A. coenophialum in these plants was the accumulation of pyrrolizidine alkaloids and conferment of resistance to the oat bird cherry aphid. A. coenophialum was reisolated from leaf sheaths of the artificially inoculated plants. Inoculations of perennial ryegrass calli with Acremonium spp. resulted in only endophyte-free plants upon regeneration of callus. We were unable to induce shoot regeneration in Acremonium-inoculated bluegrass and orchardgrass calli." |
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| Language: | English |
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| References: | 18 |
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| Note: | Tables
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Johnson, M. C., L. P. Bush, and M. R. Siegel. 1986. Infection of tall fescue with Acremonium coenophialum by means of callus culture. Plant Dis. 70(5):p. 380-382. |
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| Web URL(s):
http://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1986Articles/PlantDisease70n05_380.pdf
Last checked: 10/13/2015
Requires: PDF Reader |
| 
MSU catalog number: SB 599 .P95 |
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