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| Web URL(s): | http://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1986Articles/PlantDisease70n05_431.pdf
Last checked: 10/13/2015
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Welty, R. E.;
Azevedo, M. D.;
Cook, K. L. |
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| Author Affiliation: | USDA-ARS, Oregon State University |
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| Title: | Detecting viable Acremonium endophytes in leaf sheaths and meristems of tall fescue and perennial ryegrass |
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| Source: | Plant Disease. Vol. 70, No. 5, May 1986, p. 431-435. |
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| Publishing Information: | St. Paul, Minn., American Phytopathological Society |
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| Keywords: | TIC Keywords: Cladosporium; Festuca arundinacea; Lolium perenne; Endophytes; Acremonium; Acremonium coenophialum; Acremonium loliae; Techniques; Research; Captafol
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| Abstract/Contents: | "A method was developed to detect viable hyphae of the endophytic fungi Acremonium coenophialum or A. loliae in seedlings of tall fescue or perennial ryegrass, respectively. Captafol 4F (479 g a.i./L) at 7,489 ug a.i./ml of deionized water was used to treat seeds of two strains of tall fescue (KY-82-3G1-316 and KY-37G1-307) and perennial ryegrass (cultivar Repell). The fungicide retarded or eliminated the growth of seedborne species of Alternaria, Cladosporium, and Epicoccum but did not influence the recovery of A. coenophialum or A. loliae from seedlings. Captafol at 14,978 ug a.i./ml stunted the roots of 3-wk-old tall fescue seedlings; shoots were not affected. No phytotoxicity was observed at 7,489 and 3,745 ug a.i./ml. When used with seedling staining and microscopic examination, the method detects viable endophyte in leaf sheaths and meristems of 3-wk-old seedlings, reduced incubation time required for seedling examination, and eliminated greenhouse culture. Seed technologists were trained to detect endophyte hyphae by this technique. Several media, including potato-dextrose, cornmeal, and bran-malt agars, were suitable for growth of A. coenophialum or A. loliae in culture." |
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| Language: | English |
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| References: | 24 |
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| Note: | Tables
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Welty, R. E., M. D. Azevedo, and K. L. Cook. 1986. Detecting viable Acremonium endophytes in leaf sheaths and meristems of tall fescue and perennial ryegrass. Plant Dis. 70(5):p. 431-435. |
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| Web URL(s):
http://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1986Articles/PlantDisease70n05_431.pdf
Last checked: 10/13/2015
Requires: PDF Reader |
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MSU catalog number: SB 599 .P95 |
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