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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2002.pdf#page=34
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Brey, Christopher W.;
Gaugler, Randy |
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| Author Affiliation: | Department of Entomology, Rutgers University |
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| Title: | Enhancement of Steinernema carpocapsae desiccation tolerance by genetic improvement |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | Cook College, Rutgers, NJ: January 10-11, 2002 |
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| Source: | Proceedings of the Eleventh Annual Rutgers TurfgrassSymposium. 2002, p. 33. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Biological control; Breeding improvement; Clones; Desiccation; Heat resistance; Steinernema carpocapsae
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| Abstract/Contents: | "The entomopathogenic nematode (ENP) Steinernema carpocapsae, is an important biological control agent for a wide range of soil dwelling insect pests. However, the field efficacy of this ENP is limited by its sensitivity to high drought and salinity conditions. We report efforts to improve the desiccation tolerance of S. carpocapsae by transforming it with the trehalose-6-phosphate synthase (tps1) and glycogen synthase genes (gsy1). Trehalose-6-phosphate synthase and glycogen synthase are enzymes involved in the biosynthesis of trehalose, a disaccharide that accumulates to stabilize the lipid biomembranes in many organisms when in response to stress. To increase desiccation tolerance by genetic modification, we have cloned gene tps1 from yeast and C. elegans into expression vectors pJJ436 and pPD95.67, respectively. In addition, we also cloned gsy1 from Steinernema feltiae into expression vector pJJ436. Vector pJJ436 contained the Ce sq-1 promoter, whereas pPD95.67 contained the promoter of the tps1 Ce gene. Vectors contained gfp transformation gene which was used as a selection marker. Vector constructions (yeast: pJYe.1; C. elegans: pP67Ce.2; S. feltiae: pJTr.1) were microinjected independently into young S. carpocasae females (48 h from infective juvenile stage). Injected females were mated with noninjected males for 2-4 days and progeny were screened for gfp expression. After selecting and maintaining gfp expressing individuals for three generations, F3 progeny will be tested for desiccation tolerance." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Brey, C. W., and R. Gaugler. 2002. Enhancement of Steinernema carpocapsae desiccation tolerance by genetic improvement. Proc. Annu. Rutgers Turfgrass Symp. p. 33. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2002.pdf#page=34
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: SB 433 .R88 |
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