| |
Web URL(s): | http://journals.fcla.edu/flaent/article/viewFile/75061/72719 Last checked: 03/25/2016 Requires: PDF Reader http://www.bioone.org/bioone/?request=get-document&issn=0015-4040&volume=085&issue=01&page=0186 Last checked: 11/2005 Access conditions: Item is within a limited access website |
Publication Type:
| Report |
Author(s): | Levy, Hazel C.;
Garcia-Maruniak, Alejandra;
Maruniak, James E. |
Author Affiliation: | Department of Entomology and Nematology, University of Florida, Gainesville, FL |
Title: | Strain identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytochrome oxidase C subunit I gene |
Section: | Research papers Other records with the "Research papers" Section
|
Source: | Florida Entomologist. Vol. 85, No. 1, March 2002, p. 186-190. |
Publishing Information: | Gainesville, Florida: Florida Entomological Society |
# of Pages: | 5 |
Keywords: | TIC Keywords: Insect identification; Analytical methods; Spodoptera frugiperda; Polymerase chain reaction; Restriction fragment length polymorphism; Genetic markers
|
Abstract/Contents: | "A simple method was developed to analyze the two morphologically indistinguishable host-associated strains of the fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith). Total DNA extracted from the FAW corn and rice strains, as well as from the S. frugiperda cell line (SF9) was used to PCR amplify a 569 base pairs region of the mitochondrial gene cytochrome oxidase subunit I (COI). The amplified DNA from the Spodoptera corn strain and the SF9 cell line contained a restriction fragment length polymorphism (RFLP) marker, the MspI recognition site that was not present in the rice strain. This PCR-RFLP method does not require purification of mitochondrial DNA (mtDNA) or the use of radioactive isotopes, and differs from previous methods in that only a few nanograms of total DNA are needed to yield clear and accurate strain identification of individual insects." |
Language: | English |
References: | 32 |
Note: | Abstract also appears in Spanish Figures |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Levy, H. C., A. Garcia-Maruniak, and J. E. Maruniak. 2002. Strain identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytochrome oxidase C subunit I gene. FLA Entomol. 85(1):p. 186-190. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=80137 |
| If there are problems with this record, send us feedback about record 80137. |
| Choices for finding the above item: |
| Web URL(s): http://journals.fcla.edu/flaent/article/viewFile/75061/72719 Last checked: 03/25/2016 Requires: PDF Reader http://www.bioone.org/bioone/?request=get-document&issn=0015-4040&volume=085&issue=01&page=0186 Last checked: 11/2005 Access conditions: Item is within a limited access website |
| MSU catalog number: QL 461 .F5 |
| Find from within TIC: Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |