Full TGIF Record # 80137
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Web URL(s):http://journals.fcla.edu/flaent/article/viewFile/75061/72719
    Last checked: 03/25/2016
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http://www.bioone.org/bioone/?request=get-document&issn=0015-4040&volume=085&issue=01&page=0186
    Last checked: 11/2005
    Access conditions: Item is within a limited access website
Publication Type:
i
Report
Author(s):Levy, Hazel C.; Garcia-Maruniak, Alejandra; Maruniak, James E.
Author Affiliation:Department of Entomology and Nematology, University of Florida, Gainesville, FL
Title:Strain identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytochrome oxidase C subunit I gene
Section:Research papers
Other records with the "Research papers" Section
Source:Florida Entomologist. Vol. 85, No. 1, March 2002, p. 186-190.
Publishing Information:Gainesville, Florida: Florida Entomological Society
# of Pages:5
Keywords:TIC Keywords: Insect identification; Analytical methods; Spodoptera frugiperda; Polymerase chain reaction; Restriction fragment length polymorphism; Genetic markers
Abstract/Contents:"A simple method was developed to analyze the two morphologically indistinguishable host-associated strains of the fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith). Total DNA extracted from the FAW corn and rice strains, as well as from the S. frugiperda cell line (SF9) was used to PCR amplify a 569 base pairs region of the mitochondrial gene cytochrome oxidase subunit I (COI). The amplified DNA from the Spodoptera corn strain and the SF9 cell line contained a restriction fragment length polymorphism (RFLP) marker, the MspI recognition site that was not present in the rice strain. This PCR-RFLP method does not require purification of mitochondrial DNA (mtDNA) or the use of radioactive isotopes, and differs from previous methods in that only a few nanograms of total DNA are needed to yield clear and accurate strain identification of individual insects."
Language:English
References:32
Note:Abstract also appears in Spanish
Figures
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Levy, H. C., A. Garcia-Maruniak, and J. E. Maruniak. 2002. Strain identification of Spodoptera frugiperda (Lepidoptera: Noctuidae) insects and cell line: PCR-RFLP of cytochrome oxidase C subunit I gene. FLA Entomol. 85(1):p. 186-190.
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Web URL(s):
http://journals.fcla.edu/flaent/article/viewFile/75061/72719
    Last checked: 03/25/2016
    Requires: PDF Reader
http://www.bioone.org/bioone/?request=get-document&issn=0015-4040&volume=085&issue=01&page=0186
    Last checked: 11/2005
    Access conditions: Item is within a limited access website
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