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Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0523.2002.00684.x/epdf Last checked: 10/08/2015 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Report |
Author(s): | Li, L.;
Qu, R. |
Author Affiliation: | Department of Crop Science, North Carolina State University, Raleigh, NC |
Title: | In vitro somatic embryogenesis in turf-type bermudagrass: Roles of abscisic acid and gibberellic acid, and occurrence of secondary somatic embryogenesis |
Section: | Original papers Other records with the "Original papers" Section
|
Source: | Plant Breeding. Vol. 121, No. 2, April 2002, p. 155-158. |
Publishing Information: | Berlin: P. Parey |
# of Pages: | 4 |
Keywords: | TIC Keywords: Calli; In vitro; Somatic embryogenesis; Cynodon dactylon; Cynodon transvaalensis; Abscisic acid; Gibberellic acid; Regeneration; Scanning electron microscopy
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Cultivar Names: | Tifgreen; Savannah |
Abstract/Contents: | "Bermudagrass is an important warm-season turfgrass species that is recalcitrant in regeneration in tissue culture. In a previous report, we observed that somatic embryogenesis of immature inflorescence culture was substantially improved when low levels of 2,4-dichlorophenoxy acetic acid (1 mg/l) and 6-benzylaminopurine (BAP, 0.01 mg/l) were included in the callus induction medium. The object of this study was to further improve the culture conditions to enhance somatic embryo formation and plantlet regeneration. It was shown that the abscisic acid supplement (2 or 5 mg/l) to the above callus induction medium further enhanced somatic embryogenesis in hybrid bermudagrass (Cynodon dactylon x Cynodon transvaalensis) cv. 'Tifgreen'. The addition of gibberellic acid (0.2 mg/l) to the BAP (1 mg/l)-containing regeneration medium accelerated germination/regeneration of the somatic embryos. Secondary and repetitive somatic embryogenesis, which is rarely reported in monocots, was observed in common bermudagrass (Cynodon dactylon, cv. 'Savannah'), and a full course of such a development was captured by a periodical microphotography. Scanning electron microscopy further confirmed the observation." |
Language: | English |
References: | 24 |
Note: | Pictures, color Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Li, L., and R. Qu. 2002. In vitro somatic embryogenesis in turf-type bermudagrass: Roles of abscisic acid and gibberellic acid, and occurrence of secondary somatic embryogenesis. Plant Breed. 121(2):p. 155-158. |
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| Web URL(s): http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0523.2002.00684.x/epdf Last checked: 10/08/2015 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: SB 119 .A1 Z4 |
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