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| Author(s): | Yaneshita, M.;
Kaneko, S.;
Sasakuma, T. |
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| Author Affiliation: | Yaneshita and Sasakuma: Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Totsuka-ku, Yokohama 244, Japan; Kaneko: Biotechnology Research Center, Taisei Co., Narashino, Japan |
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| Title: | Allotetraploidy of Zoysia species with 2n = 40 based on a RFLP genetic map |
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| Source: | Theoretical and Applied Genetics. Vol. 98, No. 5, April 1999, p. 751-756. |
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| Publishing Information: | Berlin: Springer |
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| # of Pages: | 6 |
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| Keywords: | TIC Keywords: Restriction fragment length polymorphism; Zoysia; Genetics; Interspecific hybrids; Clones; Genomes; DNA; Photosynthesis; Enzymes; Alleles; Loci
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| Abstract/Contents: | "A RFLP linkage map of Zoysia spp. (2n = 40), a warm-season turfgrass, was constructed by using the self-pollinated progenies obtained from an interspecific hybrid. Out of 115 DNA clones tested, 100 (87.0%), including 55 genomic clones, 38 cDNA clones, and seven gene clones encoding photosynthetic enzymes showed allelic-RFLP banding patterns among the parental accessions. We found that 26 probes detected two or more loci segregating in the self-pollinated progenies independently. The RFLP linkage map of Zoysia spp. consists of 115 loci in 22 linkage groups ranging in size from 12.5 cM to 141.3 cM with a total map distance of 1506 cM. Six RFLP loci (5.4%) showed significant segregation distortion (P<0.01). Two loci out of six were mapped to linkage group II, and another two loci were mapped to group VII. In the RFLP linkage map of zoysiagrass, five pairs of linkage groups sharing a series of duplicated loci with approximately the same order were identified. Therefore, we conclude that Zoysia spp. with 2n = 40 should be considered as allotetraploids, which might have evolved from progenitors with a basic chromosome number of ten (x=10)." |
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| Language: | English |
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| References: | 20 |
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| Note: | Tables
Figures |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yaneshita, M., S. Kaneko, and T. Sasakuma. 1999. Allotetraploidy of Zoysia species with 2n = 40 based on a RFLP genetic map. Theor. Appl. Genet. 98(5):p. 751-756. |
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