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DOI: | 10.1017/S0953756297005340 |
Web URL(s): | https://www.sciencedirect.com/science/article/pii/S0953756208609168?via%3Dihub Last checked: 10/08/2019 Access conditions: Item is within a limited access website |
Publication Type:
| Refereed |
Author(s): | Mahuku, G. S.;
Hsiang, Tom;
Yang, L. |
Author Affiliation: | Mahuku: Agriculture and Agri-food Canada, Charlottetown Research Centre, Charlottetown, PEI; Hsiang and Yang: Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada |
Title: | Genetic diversity of Microdochium nivale isolates from turfgrass |
Source: | Mycological Research. Vol. 102, No. 5, May 1998, p. 559-567. |
Publishing Information: | Cambridge University Press |
# of Pages: | 9 |
Related Web URL: | http://www.sciencedirect.com/science/article/pii/S0953756208609168 Last checked: 06/09/2015 Notes: Abstract only |
Keywords: | TIC Keywords: Genetic diversity; Microdochium nivale; Polymerase chain reaction; Random amplified polymorphic DNA markers; Restriction fragment length polymorphism; Genotypes
|
Abstract/Contents: | "Conserved primers were used in a polymerase chain reaction to amplify the ITS region of the rDNA of 100 Microdochium nivale isolates collected from different turfgrasses in southern Ontario. The profile of the restriction digestion of the amplified ITS region revealed that all the M. nivale isolates analysed belonged to var. nivale. RAPD profiling and RFLP analyses of the IGS regions of rDNA revealed extensive genetic diversity within var. nivale. With RAPD markers, the average similarity coefficient was 66% and the estimate of genotypic diversity was 0.179. Population subdivision analysis showed that 92.2% of the total genetic diversity was found among individuals within populations compared to 7.8% among populations. In dendograms derived from genetic distances using RAPD and IGS-RFLP markers, there was some evidence for host specialization. Most RAPD markers were shared by individuals from different turfgrasses, and the populations were not highly differentiated. The high level of genotypic diversity detected within populations and the low level of genetic differentiation among populations show that recombination and migration are likely playing important roles in the population biology of M. nivale var. nivale." |
Language: | English |
References: | 40 |
Note: | Equations Pictures, b/w Tables Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Mahuku, G. S., T. Hsiang, and L. Yang. 1998. Genetic diversity of Microdochium nivale isolates from turfgrass. Mycol. Res. 102(5):p. 559-567. |
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| DOI: 10.1017/S0953756297005340 |
| Web URL(s): https://www.sciencedirect.com/science/article/pii/S0953756208609168?via%3Dihub Last checked: 10/08/2019 Access conditions: Item is within a limited access website |
| MSU catalog number: QK 600. B6 |
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