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| Web URL(s): | https://www.dl.sciencesocieties.org/publications/cs/articles/42/5/1627
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| Publication Type:
| Refereed |
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| Author(s): | Hill N. S.;
Hiatt E. E. III;
Bouton, J. H.;
Tapper, B. |
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| Author Affiliation: | Department of Crop and Soil Sciences, University of Georgia, Athens, GA |
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| Title: | Strain-specific monoclonal antibodies to a nontoxic tall fescue endophyte |
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| Section: | Forage & grazing lands
Other records with the "Forage & grazing lands" Section
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| Source: | Crop Science. Vol. 42, No. 5, September/October 2002, p. 1627-1630. |
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| Publishing Information: | Madison, WI: Crop Science Society of America |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: Antibodies; Endophytes; Festuca arundinacea; Acremonium coenophialum; Toxicity; Proteins
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| Abstract/Contents: | "Use of proprietary nontoxic endophytes that have been introduced into tall fescue provides a value-added product to livestock producers consuming cool season grass species. A need exists to have a rapid screening method to monitor seed and fields of the cool season species containing nontoxic endophytes. Neotyphodium coenophialum strain AR542, was isolated by AgResearch, New Zealand, and has been introduced into improved tall fescue (Festuca arundinacea Schreb.) monoclonal antiboides specific to AR542 and test the antibodies for cross reaction with other nontoxic endophytes and endemic toxic endophytes of tall fescue. Antigenic proteins were isloated from AR542 and vaccinated into three mice (Mus musculus). Splenic hybridoma cell lines were screened for affinity to AR542, N. coenophialum isolate EDN11,and other ascomycetes. Two cell lines expressing specificity to AR542 were identified. Comparative analysis was conducted to examine the specifity of the AR542-specific test with a nonspecific antibody test. Seed and tiller samples had endophyte values greater than 67 and 90% infection, respectively, when tested with the nonspecific test. Minor spurious false positives occurred in seed and vegetative tissues when tested with AR542-specific antibodies, but only seeds and tiller tissues containing AR542 were similar to the nonspecific test. Therefore, AR542-specific antibodies may be an efficent means to test for presence of N. coenophialum strain AR542 in tall fescue." |
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| Language: | English |
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| References: | 20 |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hill, N. S., E. E. III Hiatt, J. H. Bouton, and B. Tapper. 2002. Strain-specific monoclonal antibodies to a nontoxic tall fescue endophyte. Crop Sci. 42(5):p. 1627-1630. |
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| Web URL(s):
https://www.dl.sciencesocieties.org/publications/cs/articles/42/5/1627
Last checked: 05/05/2017
Access conditions: Item is within a limited-access website
https://www.dl.sciencesocieties.org/publications/cs/pdfs/42/5/1627
Last checked: 05/05/2017
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: SB 183 .C7 |
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