Full TGIF Record # 83853
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Web URL(s):http://www.bioone.org/bioone/?request=get-document&issn=0043-1745&volume=050&issue=06&page=0721
    Last checked: 11/2005
    Access conditions: Item is within a limited access website
Publication Type:
i
Refereed
Author(s):Baerson, Scott R.; Rodriguez, Damian J.; Biest, Nancy A.; Tran, Minhtien; You, Jinsong; Kreuger, Roger W.; Dill, Gerald M.; Pratley, James E.; Gruys, Kenneth J.
Author Affiliation:Baerson, Rodriguez, Biest, Tran, You, Kreuger, Dill, Gruys: Monsanto Company, St. Louis, MO; and Pratley:Farrer Centre, Faculty of Science and Agriculture, Charles Sturt University, Wagga Wagga, New South Wales, Australia
Title:Investigating the mechanism of glyphosate resistance in rigid ryegrass (Lolium rigidum)
Section:Physiology, chemistry and biochemistry
Other records with the "Physiology, chemistry and biochemistry" Section
Source:Weed Science. Vol. 50, No. 6, November/December 2002, p. 721-730.
Publishing Information:Champaign, IL: Weed Science Society of America
# of Pages:10
Keywords:TIC Keywords: Glyphosate; Lolium rigidum; Herbicide resistance; Genes; Restriction fragment length polymorphism; DNA; Ribonucleic acid; Genetic resistance
Abstract/Contents:"Glyphosate is a broad-spectrum herbicide that has been used extensively for more than 20 yr. The first glyphosate-resistant biotype appeared in 1996; it involved a rigid ryegrass population from Australia that exhibited an LD50 value approximately 10-fold higher than that of sensitive biotypes. We have characterized gene expression levels and glyphosate sensitivity of 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS), the target enzyme for glyphosate inhibition, in sensitive and resistant lines derived from this population. Restriction fragment length polymorphism analyses were also performed to examine the distribution of EPSPS gene variants and the gene copy number. A two- to threefold increase in basal EPSPS messenger RNA (mRNA) and enzyme activity levels was observed in the most resistant lines analyzed; however, differences among lines in the sensitivity of EPSPS to glyphosate were not apparent. Induction of EPSPS was observed within 48 h after application of 1.5 kg ae ha-1 of glyphosate. This was reflected in elevated levels of both EPSPS mRNA and enzyme activity. Similarly, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase mRNA levels increased after glyphosate treatment; however, basal and induced transcript levels were comparable for sensitive and resistant lines in this case. The restriction fragment length polymorphism analyses showed no evidence for gene amplification or cosegregation of a specific EPSPS gene variant with glyphosate resistance. EPSPS expression in lines exhibiting an intermediate level of resistance was indistinguishable from that in glyphosate-sensitive lines, suggesting that the mechanism could, at least in part, be non-target-based."
Language:English
References:38
Note:Figures
Tables
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Baerson, S. R., D. J. Rodriguez, N. A. Biest, M. Tran, J. You, R. W. Kreuger, et al. 2002. Investigating the mechanism of glyphosate resistance in rigid ryegrass (Lolium rigidum). Weed Sci. 50(6):p. 721-730.
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Web URL(s):
http://www.bioone.org/bioone/?request=get-document&issn=0043-1745&volume=050&issue=06&page=0721
    Last checked: 11/2005
    Access conditions: Item is within a limited access website
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MSU catalog number: SB 610 .W38
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